Induction of Specific Humoral Immune Response in Mice against a Pseudomonas aeruginosa Chimeric PilQ/PilA Protein

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GHOLAMI MEHRDAD; SALIMI CHIRANI ALIREZA; FALAK REZA; MOSHIRI MONA; RAZAVI SHABNAM; IRAJIAN GHOLAMREZA

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Journal: REPORTS OF BIOCHEMISTRY AND MOLECULAR BIOLOGY Year:2018 | Volume:7 | Issue:1 Page(s): 38-44

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Title

Induction of Specific Humoral Immune Response in Mice against a Pseudomonas aeruginosa Chimeric PilQ/PilA Protein

Author(s)

Keywords

Chimeric protein

PilQ

Pseudomonas aeruginosa

Type IV pili

Vaccine

Abstract

Background: Pseudomonas aeruginosa, an opportunistic pathogen, is a common cause of healthcare-associated infections in immunocompromised individuals. The rapid emergence of multidrug-resistant strains has made P. aeruginosa infections progressively difficult to treat. In this study we evaluated the effect of a Chimeric protein containing a P. aeruginosa PilQ fragment and the PilA disulfide loop (PilA-DSL) on the humoral immune response in BALB/c mice. Methods: A chimeric gene encoding an immunogenic region of PilQ and the PilA-DSL was synthesized. Following bacterial expression and purification, the protein was administered to mice and the humoral immune response analyzed. The resulting antibodies were analyzed using an opsonophagocytic killing assay. Results: The anti-recombinant protein antibody titer was significantly greater in immunized mice than in controls. In addition, antibody titers were significantly increased after booster immunizations, and the immunizations induced opsonophagocytosis of P. aeruginosa PAO1. Conclusions: These results suggest that an anti-adhesion-based vaccination may be effective in preventing P. aeruginosa infections. Further studies are needed to evaluate the abilities of such bivalent proteins to induce strong immune responses.

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APA: Copy

GHOLAMI, MEHRDAD, SALIMI CHIRANI, ALIREZA, FALAK, REZA, MOSHIRI, MONA, RAZAVI, SHABNAM, & IRAJIAN, GHOLAMREZA. (2018). Induction of Specific Humoral Immune Response in Mice against a Pseudomonas aeruginosa Chimeric PilQ/PilA Protein. REPORTS OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, 7(1), 38-44. SID. https://sid.ir/paper/767592/en

Vancouver: Copy

GHOLAMI MEHRDAD, SALIMI CHIRANI ALIREZA, FALAK REZA, MOSHIRI MONA, RAZAVI SHABNAM, IRAJIAN GHOLAMREZA. Induction of Specific Humoral Immune Response in Mice against a Pseudomonas aeruginosa Chimeric PilQ/PilA Protein. REPORTS OF BIOCHEMISTRY AND MOLECULAR BIOLOGY[Internet]. 2018;7(1):38-44. Available from: https://sid.ir/paper/767592/en

IEEE: Copy

MEHRDAD GHOLAMI, ALIREZA SALIMI CHIRANI, REZA FALAK, MONA MOSHIRI, SHABNAM RAZAVI, and GHOLAMREZA IRAJIAN, “Induction of Specific Humoral Immune Response in Mice against a Pseudomonas aeruginosa Chimeric PilQ/PilA Protein,” REPORTS OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, vol. 7, no. 1, pp. 38–44, 2018, [Online]. Available: https://sid.ir/paper/767592/en

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