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Information Journal Paper

Title

Effect of UV-Photofunctionalization on Bioactivity of Titanium to Promote Human Mesenchymal Stem Cells

Pages

  7-11

Abstract

 Background and Aim: The present study introduces photofunctionalization as a technique for tackling biological aging and increasing the bioactivity of Titanium. This in-vitro study evaluated the effects of ultraviolet (UVC) light treatment of Titanium surfaces with different time-related changes on the behavior and function of human Mesenchymal Stem Cells (MSCs). Materials and Methods: MSCs were cultured on nine untreated Titanium surfaces (four-week-old surfaces; group 1), nine fresh UVC-treated surfaces (immediately after UV treatment; group 2), and nine three-day-old UVC-treated surfaces (group 3). Cellular proliferation and attachment were measured by MTT. Alkaline phosphatase (ALP) was assessed by total protein extraction, and the enzyme activity was evaluated using a special ALP kit. One-way analysis of variance (ANOVA) and post-hoc Tukey’ s test were used to examine the effect of UVC on Titanium surfaces. Results: The mean attachment of MSCs to Titanium disks in groups 1 to 3 was 0. 118± 0. 003, 0. 103± 0. 007, and 0. 155± 0. 009, respectively. The mean proliferation of MSCs in groups 1 to 3 was 0. 229± 0. 004, 0. 189± 0. 023, and 0. 298± 0. 020, respectively. The proliferation and attachment in group 3 were significantly higher compared to other groups (P<0. 05). Speeds of MSCs growth in groups 1 to 3 were 94%, 81%, and 92%, respectively. The ALP activity of MSCs in groups 1 to 3 was 0. 153± 0. 003, 0. 187± 0. 003, and 0. 161± 0. 003, respectively. The ALP activity in group 2 was significantly higher compared to other groups (P<0. 05). Conclusion: UVC pretreatment of Titanium surfaces increases the ALP activity. However, cellular attachment and proliferation were not increased in the present study due to the high probability of laboratory error.

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    APA: Copy

    Shbahangfar, Mohammad Reza, JAFARI, EHSAN, Tarashohi, Anahita, Olyaee, P., & Bitaraf, Tahereh. (2019). Effect of UV-Photofunctionalization on Bioactivity of Titanium to Promote Human Mesenchymal Stem Cells. JOURNAL OF RESEARCH IN DENTAL AND MAXILLOFACIAL SCIENCES, 4(2), 7-11. SID. https://sid.ir/paper/767778/en

    Vancouver: Copy

    Shbahangfar Mohammad Reza, JAFARI EHSAN, Tarashohi Anahita, Olyaee P., Bitaraf Tahereh. Effect of UV-Photofunctionalization on Bioactivity of Titanium to Promote Human Mesenchymal Stem Cells. JOURNAL OF RESEARCH IN DENTAL AND MAXILLOFACIAL SCIENCES[Internet]. 2019;4(2):7-11. Available from: https://sid.ir/paper/767778/en

    IEEE: Copy

    Mohammad Reza Shbahangfar, EHSAN JAFARI, Anahita Tarashohi, P. Olyaee, and Tahereh Bitaraf, “Effect of UV-Photofunctionalization on Bioactivity of Titanium to Promote Human Mesenchymal Stem Cells,” JOURNAL OF RESEARCH IN DENTAL AND MAXILLOFACIAL SCIENCES, vol. 4, no. 2, pp. 7–11, 2019, [Online]. Available: https://sid.ir/paper/767778/en

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