Epidemiology and Identification of Actin Gene of Trichomonas vaginalis Genotypes in Women of Southeast of Iran Using PCR-RFLP

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Shahraki Fateme; FOULADI BAHMAN; SALIMI KHORASHAD ALIREZA; SEPEHRI RAD NAHID; DABIRZADEH MANSOUR

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Journal: Crescent Journal of Medical and Biological Sciences Year:2020 | Volume:7 | Issue:1 Page(s): 82-90

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Title

Epidemiology and Identification of Actin Gene of Trichomonas vaginalis Genotypes in Women of Southeast of Iran Using PCR-RFLP

Author(s)

Keywords

Trichomonas vaginalis

Actin gene

Zahedan

Zabol

Epidemiology

Abstract

Objectives: C Trichomoniasis is one of the most common sexually transmitted diseases in the world, which is caused by Trichomonas vaginalis. It is also the most commonly reported sexually transmitted disease after the viral infections, which affects around 180 million people around the world each year. The people infected with this parasite exhibit a wide range of symptoms. To the best of our knowledge the genetic variation, prevalence and related factors affecting the disease have not been well studied. Therefore, this study aimed to determine the prevalence of T. vaginalis in women of southeast of Iran. Materials and Methods: Out of 500 patient women referred to the hospitals of Imam Khomeini in Zabol and Ali Ibn Abi Talib (AS) in Zahedan, 25 positive clinical samples were isolated from vaginal discharge and urine by culture method during June 2015 and May 2016. First, DNA was extracted and then all samples were subjected to nested PCR. Six different genotypes of Actin gene were identified by PCR-RFLP in Trichomonas vaginalis in Zahedan and Zabol. All PCR products were digested with HindII, RsaI, and MesI restriction enzymes. All participants completed a questionnaire recommended by gynecologists and midwifery experts. Results: As a result, the genotypes of H, G, E, I, and N were identified in this study, from which the genotype E was the dominant genotype of T. vaginalis in Zahedan and Zabol. There was also a significant association between the type of clinical symptoms and the level of infection (P=0. 0001). Conclusions: To sum up, disease as a health problem must be controlled through epidemiologic and genetic methods. Moreover, controlling the disease is closely associated with education and drug resistance or sensitivity related to genetic variation and epidemiologic factors.

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References

Considerable Genetic Diversity of Trichomonas vaginalis Clinical Isolates in a Targeted Population in South of Iran

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APA: Copy

Shahraki, Fateme, FOULADI, BAHMAN, SALIMI KHORASHAD, ALIREZA, SEPEHRI RAD, NAHID, & DABIRZADEH, MANSOUR. (2020). Epidemiology and Identification of Actin Gene of Trichomonas vaginalis Genotypes in Women of Southeast of Iran Using PCR-RFLP. CRESCENT JOURNAL OF MEDICAL AND BIOLOGICAL SCIENCES, 7(1), 82-90. SID. https://sid.ir/paper/774013/en

Vancouver: Copy

Shahraki Fateme, FOULADI BAHMAN, SALIMI KHORASHAD ALIREZA, SEPEHRI RAD NAHID, DABIRZADEH MANSOUR. Epidemiology and Identification of Actin Gene of Trichomonas vaginalis Genotypes in Women of Southeast of Iran Using PCR-RFLP. CRESCENT JOURNAL OF MEDICAL AND BIOLOGICAL SCIENCES[Internet]. 2020;7(1):82-90. Available from: https://sid.ir/paper/774013/en

IEEE: Copy

Fateme Shahraki, BAHMAN FOULADI, ALIREZA SALIMI KHORASHAD, NAHID SEPEHRI RAD, and MANSOUR DABIRZADEH, “Epidemiology and Identification of Actin Gene of Trichomonas vaginalis Genotypes in Women of Southeast of Iran Using PCR-RFLP,” CRESCENT JOURNAL OF MEDICAL AND BIOLOGICAL SCIENCES, vol. 7, no. 1, pp. 82–90, 2020, [Online]. Available: https://sid.ir/paper/774013/en

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