مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

104
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

56
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

Construction of a Prokaryotic Expression Vector harboring Two HIV-1 Accessory Genes

Pages

  11-17

Abstract

 Background and objectives: HIV-1 Nef and Vpr antigens have been described as suitable candidates for therapeutic HIV vaccine development. The aim of this study was to generate Nef-Vpr fusion gene construct and to clone the construct into pET-23a (+), a prokaryotic expression vector. Methods: HIV-1 Nef and Vpr genes were PCR-amplified from the pNL4-3 plasmid using specific primers and Pfu DNA polymerase. Results of PCR amplification were visualized by electrophoresis on 0. 8% agarose gel. At first, the amplified Nef fragment was cloned into NheI and BamHI restriction sites of pET-23a expression vector. Next, cloning of Vpr gene was performed into BamHI and HindIII restriction sites of the pET-23a-Nef vector. Finally, purity of the recombinant pET-23-Nef-Vpr construct was determined by NanoDrop spectrophotometry. Results: PCR amplification of Nef and Vpr genes was confirmed by detection of 620 bp and 291 bp bands, respectively. Cloning of the Nef-Vpr construct into the vector was confirmed by detection of a 911 bp fragment following enzymatic digestion with NheI and HindIII and sequencing. Conclusion: The successful construction of recombinant fusion plasmid encoding a chimeric Nef-Vpr gene was performed in a prokaryotic expression vector for development of HIV-1 recombinant protein vaccine in near future.

Cites

  • No record.

    • References

    Cite

    APA: Copy

    Nikyar, Arash, BOLHASSANI, AZAM, Rouhollah, Fatemeh, & Heshmati, Masoumeh. (2021). Construction of a Prokaryotic Expression Vector harboring Two HIV-1 Accessory Genes. MEDICAL LABORATORY JOURNAL, 15(2), 11-17. SID. https://sid.ir/paper/777233/en

    Vancouver: Copy

    Nikyar Arash, BOLHASSANI AZAM, Rouhollah Fatemeh, Heshmati Masoumeh. Construction of a Prokaryotic Expression Vector harboring Two HIV-1 Accessory Genes. MEDICAL LABORATORY JOURNAL[Internet]. 2021;15(2):11-17. Available from: https://sid.ir/paper/777233/en

    IEEE: Copy

    Arash Nikyar, AZAM BOLHASSANI, Fatemeh Rouhollah, and Masoumeh Heshmati, “Construction of a Prokaryotic Expression Vector harboring Two HIV-1 Accessory Genes,” MEDICAL LABORATORY JOURNAL, vol. 15, no. 2, pp. 11–17, 2021, [Online]. Available: https://sid.ir/paper/777233/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button