EFFECT OF MINERALIZED BONE ALLOGRAFT SCAFFOLD WITH COVERAGE OF UCB-DERIVED STROMAL CELLS ON EX VIVO EXPANSION OF CD34+ CELLS

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HASHEMI Z.S.; FOROUZANDEH MOGHADAM M.; SOLEIMANI M.; KHAMISIPOUR GH.; MOSSAHEBI MOHAMMADI M.

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Journal: The Scientific Journal of Iranian Blood Transfusion Year:2012 | Volume:9 | Issue:3 (36) Page(s): 199-213

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Title

EFFECT OF MINERALIZED BONE ALLOGRAFT SCAFFOLD WITH COVERAGE OF UCB-DERIVED STROMAL CELLS ON EX VIVO EXPANSION OF CD34+ CELLS

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Keywords

MBAQ3

HEMATOPOIETIC STEM CELLSQ3

UMBILICAL CORD BLOODQ3

ALLOGRAFTQ3

Abstract

Background and Objectives: Bone marrow transplantation with UMBILICAL CORD BLOOD (UCB) in adult recipients is limited mainly by a low CD34+ cell dose. To overcome this shortcoming by minimum manipulation, mineralized bone ALLOGRAFT (MBA) scaffold coated by UCB-USSC (unrestricted somatic stem cells) was used to expand CD34+ cells from UCB.Materials and Methods: In this experimental study, UCB-USSCs were isolated and characterized by morphologic and immunophenotypical analysis. Then they were seeded on MBA (for 3D figuration) and culture plate (for 2D figuration) as a feeder layer. CD34+ cells were isolated from the UCB (by MACS method) and were expanded in 2D and 3D conditions for 3 weeks. At the end, cell count, flow cytometry, cologenic assay, and LTC-IC were done.Results: After 3 weeks, ex vivo expansion of UCB-CD34+ was enhanced 250±13.2 in 3D. The highest CFC expansion and LTC-IC were observed at day 14. Flow cytometry analysis showed the lowest percentage in 3D culture.Conclusions: USSCs by 3D MBA structure produce the hematopoietic cytokine, extracellular matrix and binding molecules, they also create the intercellular interaction that can be used as a suitable feeder layer. It provides anex vivo mimicry of bone marrow niche by enhancing surface/volume ratio. This model could be a suitable and alternative environment for HSCs expansion and hematopoiesis.

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APA: Copy

HASHEMI, Z.S., FOROUZANDEH MOGHADAM, M., SOLEIMANI, M., KHAMISIPOUR, GH., & MOSSAHEBI MOHAMMADI, M.. (2012). EFFECT OF MINERALIZED BONE ALLOGRAFT SCAFFOLD WITH COVERAGE OF UCB-DERIVED STROMAL CELLS ON EX VIVO EXPANSION OF CD34⁺CELLS. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), 9(3 (36)), 199-213. SID. https://sid.ir/paper/78322/en

Vancouver: Copy

HASHEMI Z.S., FOROUZANDEH MOGHADAM M., SOLEIMANI M., KHAMISIPOUR GH., MOSSAHEBI MOHAMMADI M.. EFFECT OF MINERALIZED BONE ALLOGRAFT SCAFFOLD WITH COVERAGE OF UCB-DERIVED STROMAL CELLS ON EX VIVO EXPANSION OF CD34⁺CELLS. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON)[Internet]. 2012;9(3 (36)):199-213. Available from: https://sid.ir/paper/78322/en

IEEE: Copy

Z.S. HASHEMI, M. FOROUZANDEH MOGHADAM, M. SOLEIMANI, GH. KHAMISIPOUR, and M. MOSSAHEBI MOHAMMADI, “EFFECT OF MINERALIZED BONE ALLOGRAFT SCAFFOLD WITH COVERAGE OF UCB-DERIVED STROMAL CELLS ON EX VIVO EXPANSION OF CD34⁺CELLS,” THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), vol. 9, no. 3 (36), pp. 199–213, 2012, [Online]. Available: https://sid.ir/paper/78322/en

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