Expression Analysis of Circulating miR-21 in Iranian Patients with Hereditary Colorectal Cancer

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AREFI MOHAMMAD; ABDOLLAHI ABBAS; KHOSRAVI AYYOOB; Moradi Abdolavahab; AGHAEE BAKHTIARI SEYED HAMID; Javid Najmeh; Evazalipour Mehdi; KIANMEHR ANVARSADAT

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Journal: MEDICAL LABORATORY JOURNAL Year:2021 | Volume:15 | Issue:2 Page(s): 1-4

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Title

Expression Analysis of Circulating miR-21 in Iranian Patients with Hereditary Colorectal Cancer

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Keywords

Colorectal cancer

Gene Expression

Real-Time Polymerase Chain Reaction

Abstract

Background and objectives: Colorectal cancer (CRC) is one of the leading causes of cancer-related mortality in the world. MicroRNAs (miRNAs) have potential as diagnostic biomarkers for various diseases including cancer. This study was undertaken to investigate expression of miR-21 before and after surgery in patients with hereditary CRC. Methods: After collecting blood samples from 39 patients and 39 healthy controls, total RNA was extracted by the TRIzol method. Following cDNA synthesis, expression of miR-21 in serum of subjects was evaluated using real-time PCR, along with two reference genes, let-7d and let-7g. The real-time expression results and Ct values were collected and analyzed based on the 2-Δ Δ ct method. Results: In spite of tumor removal, serum miR-21 expression levels was significantly higher in hereditary CRC patients compared with controls (P=0. 022). Conclusion: Our results confirmed that samples from hereditary cases of CRC must not be included in experiments on the diagnostic potential of miRNAs.

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Post-colonoscopy colorectal cancer: the key role of molecular pathological epidemiology

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APA: Copy

AREFI, MOHAMMAD, ABDOLLAHI, ABBAS, KHOSRAVI, AYYOOB, Moradi, Abdolavahab, AGHAEE BAKHTIARI, SEYED HAMID, Javid, Najmeh, Evazalipour, Mehdi, & KIANMEHR, ANVARSADAT. (2021). Expression Analysis of Circulating miR-21 in Iranian Patients with Hereditary Colorectal Cancer. MEDICAL LABORATORY JOURNAL, 15(2), 1-4. SID. https://sid.ir/paper/785369/en

Vancouver: Copy

AREFI MOHAMMAD, ABDOLLAHI ABBAS, KHOSRAVI AYYOOB, Moradi Abdolavahab, AGHAEE BAKHTIARI SEYED HAMID, Javid Najmeh, Evazalipour Mehdi, KIANMEHR ANVARSADAT. Expression Analysis of Circulating miR-21 in Iranian Patients with Hereditary Colorectal Cancer. MEDICAL LABORATORY JOURNAL[Internet]. 2021;15(2):1-4. Available from: https://sid.ir/paper/785369/en

IEEE: Copy

MOHAMMAD AREFI, ABBAS ABDOLLAHI, AYYOOB KHOSRAVI, Abdolavahab Moradi, SEYED HAMID AGHAEE BAKHTIARI, Najmeh Javid, Mehdi Evazalipour, and ANVARSADAT KIANMEHR, “Expression Analysis of Circulating miR-21 in Iranian Patients with Hereditary Colorectal Cancer,” MEDICAL LABORATORY JOURNAL, vol. 15, no. 2, pp. 1–4, 2021, [Online]. Available: https://sid.ir/paper/785369/en

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