EVALUATION OF THE EFFECTS OF MIP1-Α ON EX VITRO EXPANSION OF HEMATOPOIETIC PROGENITOR CELLS IN DIFFERENT CULTURE MEDIA FOR THE PURPOSE OF CORD BLOOD TRANSPLANTATION

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ALI MOGHADAM K.; KHALILI M.; SOLEYMANI M.; GHODSI P.; MOHYEDIN M.; MOEZI LEYLI; ARJMAND A.; GHAVAMZADEH ARDESHIR; HAYAT P.

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Journal: The Scientific Journal of Iranian Blood Transfusion Year:2006 | Volume:3 | Issue:2 Page(s): 145-152

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Title

EVALUATION OF THE EFFECTS OF MIP1-Α ON EX VITRO EXPANSION OF HEMATOPOIETIC PROGENITOR CELLS IN DIFFERENT CULTURE MEDIA FOR THE PURPOSE OF CORD BLOOD TRANSPLANTATION

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Keywords

MIP1-ΑQ4

CORD BLOODQ4

EX VIVO EXPANSIONQ2

HEMATOPOIETIC PROGENITOR CELLSQ2

Abstract

Background and Objectives: Umbilical CORD BLOOD (CB) has been identified both as a rich source for hematopoietic stem cells (HSCs) and as an alternative to bone marrow transplantation. To accelerate the pace of engraftment in CORD BLOOD (CB) transplantation, HSCs should be ex vivo expanded. So the aim of this study was to evaluate the effects of macrophage inflammatory protein-1 alpha (MIP1- α) on EX VIVO EXPANSION of HSCs and its role in prevention of HSCs differentiation in different conditions.Materials and Methods: Mononuclear cells (MNCs) were seperated from CORD BLOOD and cultured in RPMI 1640 with 10% fetal calf serum (FCS) or 10% CORD BLOOD plasma (CBP) or serum free media (SF). Culture media contained 50 ng/ml of interlukin 6 (IL-6), interlukin 3 (IL-3), thrombopoietin (TPO), stem cell factor (SCF), and flt3-ligand. MNCs were cultured for 2 weeks in the presence or absence of MIP1-Α in CO2 incubator; expansion potential was then studied by MNCs counting and flowcytometry for CD34+/CD38- cells in 7 and 14 days.Results: In all MIP1-Α contained media, more increase in expansion of CD34+/CD38- and CD34+ cells was observed.Conclusions: These results suggest that the effect of MIP1- α depends on type of medium and the cytokines present in the culture; it also shows that addition of MIP1-Α is useful for clinically EX VIVO EXPANSION of CORD BLOOD-derived progenitor cells.

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EX VIVO-EXPANSION OF CORD BLOOD CELLS AND ITS CLINICAL APPLICATION

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APA: Copy

ALI MOGHADAM, K., KHALILI, M., SOLEYMANI, M., GHODSI, P., MOHYEDIN, M., MOEZI, LEYLI, ARJMAND, A., GHAVAMZADEH, ARDESHIR, & HAYAT, P.. (2006). EVALUATION OF THE EFFECTS OF MIP1-Α ON EX VITRO EXPANSION OF HEMATOPOIETIC PROGENITOR CELLS IN DIFFERENT CULTURE MEDIA FOR THE PURPOSE OF CORD BLOOD TRANSPLANTATION. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), 3(2), 145-152. SID. https://sid.ir/paper/78624/en

Vancouver: Copy

ALI MOGHADAM K., KHALILI M., SOLEYMANI M., GHODSI P., MOHYEDIN M., MOEZI LEYLI, ARJMAND A., GHAVAMZADEH ARDESHIR, HAYAT P.. EVALUATION OF THE EFFECTS OF MIP1-Α ON EX VITRO EXPANSION OF HEMATOPOIETIC PROGENITOR CELLS IN DIFFERENT CULTURE MEDIA FOR THE PURPOSE OF CORD BLOOD TRANSPLANTATION. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON)[Internet]. 2006;3(2):145-152. Available from: https://sid.ir/paper/78624/en

IEEE: Copy

K. ALI MOGHADAM, M. KHALILI, M. SOLEYMANI, P. GHODSI, M. MOHYEDIN, LEYLI MOEZI, A. ARJMAND, ARDESHIR GHAVAMZADEH, and P. HAYAT, “EVALUATION OF THE EFFECTS OF MIP1-Α ON EX VITRO EXPANSION OF HEMATOPOIETIC PROGENITOR CELLS IN DIFFERENT CULTURE MEDIA FOR THE PURPOSE OF CORD BLOOD TRANSPLANTATION,” THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), vol. 3, no. 2, pp. 145–152, 2006, [Online]. Available: https://sid.ir/paper/78624/en

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