CMV DETECTION AND MONITORING IN BONE MARROW TRANSPLANT (BMT) RECIPIENTS BY REAL-TIME PCR (RQ-PCR)

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OBEIDI N.; GHARAH BAGHIAN A.; GHAFFARI H.; ALI MOGHADAM K.; GHAVAMZADEH ARDESHIR; SHAMSHIRI A.; DEHGHAN M.

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Journal: THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON) Year:2007 | Volume:4 | Issue:1 Page(s): 1-9

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Title

CMV DETECTION AND MONITORING IN BONE MARROW TRANSPLANT (BMT) RECIPIENTS BY REAL-TIME PCR (RQ-PCR)

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Keywords

CYTOMEGALOVIRUS (CMV)Q4

BONE MARROW TRANSPLANT (BMT)Q4

PCRQ3

Abstract

Background and Objectives: Cytomegalovirus (CMV) is an important pathogen in patients undergoing bone marrow transplantation. The prevalence of CMV varies from 30-100% in different countries as shown by seroepidemiological studies. Only 20-25% of patients develop CMV disease. Because of the similarity between CMV and GVHD and the different therapies required, detection of viral load will be effective in patients' survival.Materials and Methods: 51 recipients of BMT were monitored for 100 days post-BMT during which the samples were collected weekly. The Real-Time PCR was developed for quantitation of CMV viral DNA, using TaqMan technology. For generation of standard curve, UL83 gene from CMV was cloned into a plasmid using a T/A cloning procedure. RQ-PCR assay was preformed in parallel with pp65 antigenemia assay on 415 samples.Results: The results obtained by both techniques were significantly correlated (p<0.01). We could detect 13×101 -15×107 (CMV DNA copies/2×105 cells) by RQ-PCR method. About 76% of patients developed at least one episode of CMV reactivation. First positive result of RQ-PCR appeared 13 days earlier than of pp65 antigenemia. After preemptive therapy, 16 days were required to achieve negative result by RQ-PCR.Conclusions: Both assays were highly correlated; however, RQ-PCR was more sensitive than the antigenemia assay. After preemptive therapy, negative results of RQ-PCR were the best indicator to determine the endpoint of treatment and its success.

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APA: Copy

OBEIDI, N., GHARAH BAGHIAN, A., GHAFFARI, H., ALI MOGHADAM, K., GHAVAMZADEH, ARDESHIR, SHAMSHIRI, A., & DEHGHAN, M.. (2007). CMV DETECTION AND MONITORING IN BONE MARROW TRANSPLANT (BMT) RECIPIENTS BY REAL-TIME PCR (RQ-PCR). THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), 4(1), 1-9. SID. https://sid.ir/paper/78733/en

Vancouver: Copy

OBEIDI N., GHARAH BAGHIAN A., GHAFFARI H., ALI MOGHADAM K., GHAVAMZADEH ARDESHIR, SHAMSHIRI A., DEHGHAN M.. CMV DETECTION AND MONITORING IN BONE MARROW TRANSPLANT (BMT) RECIPIENTS BY REAL-TIME PCR (RQ-PCR). THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON)[Internet]. 2007;4(1):1-9. Available from: https://sid.ir/paper/78733/en

IEEE: Copy

N. OBEIDI, A. GHARAH BAGHIAN, H. GHAFFARI, K. ALI MOGHADAM, ARDESHIR GHAVAMZADEH, A. SHAMSHIRI, and M. DEHGHAN, “CMV DETECTION AND MONITORING IN BONE MARROW TRANSPLANT (BMT) RECIPIENTS BY REAL-TIME PCR (RQ-PCR),” THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), vol. 4, no. 1, pp. 1–9, 2007, [Online]. Available: https://sid.ir/paper/78733/en

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