THE EFFECT OF TELOMERASE INHIBITOR MST-312 ON APOPTOSIS OF ACUTE LYMPHOBLASTIC LEUKEMIA CELL LINE NALM-6

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GHASEMIMEHR N.; YAZDANI Z.; FARSINEJAD A.R.; MIRZAEE KHALILABADI R.; FATEMI A.

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Journal: The Scientific Journal of Iranian Blood Transfusion Year:2018 | Volume:15 | Issue:2 Page(s): 120-132

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Title

THE EFFECT OF TELOMERASE INHIBITOR MST-312 ON APOPTOSIS OF ACUTE LYMPHOBLASTIC LEUKEMIA CELL LINE NALM-6

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Keywords

TELOMERASEQ2

APOPTOSISQ2

ACUTE LYMPHOID LEUKEMIAQ2

Abstract

Background and Objectives: There have been continuous efforts in TELOMERASE-targeted therapy for cancer treatment because TELOMERASE expression is significantly increased in about 85- 90% of human cancers while its expression is usually silenced in normal cells.Materials and Methods: Throughout this experimental study, NALM-6 cells were treated with different concentrations of TELOMERASE inhibitor, MST-312 at different times. Then, cell viability and metabolic activity were evaluated by trypan blue and MTT assays; cell APOPTOSIS was also evaluated by flow cytometry using Annexin V APOPTOSIS Detection Kit. In addition, the expression of BAX and BCL2 genes was evaluated in the treated cells using Quantitative Real-time PCR. Results: A dose-and time-dependent decrease was observed in the viability of NALM-6 cells after exposure with different concentration of MST-312. Over 50% decrease was observed in the viability of the cells treated with 8 mM of MST-312 after 48 h. The cytotoxic effect of MST-312 was dose- dependent, with approximately 6, 19.69 and 56.9% reduction in metabolic activity of NALM-6 cells after 48 h exposure with 2, 4, and 8 mM of MST-312, respectively. Approximately 20% APOPTOSIS was observed in NALM-6 cells treated with 4mM of MST-312 after 48 h. Gene expression analysis also showed that 4mM of MST-312 led to upregulation of BAX and downregulation of Bcl-2 genes Conclusions Inhibition of TELOMERASE activity by MST-312 can be proposed as a new candidate for the treatment of acute lymphoblastic leukemia.

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APA: Copy

GHASEMIMEHR, N., YAZDANI, Z., FARSINEJAD, A.R., MIRZAEE KHALILABADI, R., & FATEMI, A.. (2018). THE EFFECT OF TELOMERASE INHIBITOR MST-312 ON APOPTOSIS OF ACUTE LYMPHOBLASTIC LEUKEMIA CELL LINE NALM-6. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), 15(2 ), 120-132. SID. https://sid.ir/paper/78759/en

Vancouver: Copy

GHASEMIMEHR N., YAZDANI Z., FARSINEJAD A.R., MIRZAEE KHALILABADI R., FATEMI A.. THE EFFECT OF TELOMERASE INHIBITOR MST-312 ON APOPTOSIS OF ACUTE LYMPHOBLASTIC LEUKEMIA CELL LINE NALM-6. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON)[Internet]. 2018;15(2 ):120-132. Available from: https://sid.ir/paper/78759/en

IEEE: Copy

N. GHASEMIMEHR, Z. YAZDANI, A.R. FARSINEJAD, R. MIRZAEE KHALILABADI, and A. FATEMI, “THE EFFECT OF TELOMERASE INHIBITOR MST-312 ON APOPTOSIS OF ACUTE LYMPHOBLASTIC LEUKEMIA CELL LINE NALM-6,” THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), vol. 15, no. 2 , pp. 120–132, 2018, [Online]. Available: https://sid.ir/paper/78759/en

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