IDENTIFICATION OF FASCIOLA SPECIES BY PCR-RFLP ASSAY IN NORTHERN IRAN

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

HALAKOU A.; KHAZAN H.; BANDEHPOUR M.; TAGHIPOUR N.; KAZEMI B.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF GORGAN UNIVERSITY OF MEDICAL SCIENCES Year:2017 | Volume:19 | Issue:3 (63) Page(s): 99-104

Download Full-Text

Persian Verion

View:

1,289

Download:

Cites:

Information Journal Paper

Title

IDENTIFICATION OF FASCIOLA SPECIES BY PCR-RFLP ASSAY IN NORTHERN IRAN

Author(s)

Keywords

LIVERQ2

SHEEPQ2

CATTLEQ2

FASCIOLA GIGANTICAQ2

FASCIOLA HEPATICAQ1

RFLP-PCRQ2

Abstract

Background and Objective: Identification of Fasciola species is important. Fascioliasis is one of the important diseases in animals and humans caused by genus Fasciola. This study was done to determine the identification of Fasciola species with RFLP-PCR in animal LIVER in Gorgan City, northern Iran.Methods: In this descriptive study, worms were obtained from the LIVERs of infected SHEEP and CATTLE in Gorgan slaughterhouse in northern Iran. DNA of worms was extracted with phenol- chloroform method. Fragment of ITS-1 genome was amplified and TasI enzyme was utilized for amplified fragments then 8 samples were sequenced.Results: A total of 49 Fasciola worms were isolated from infected CATTLE and SHEEP. The PCR products of all specimens were affected by the TasI enzyme, and F.hepatica species showed two fragments and F.gigantica species indicated three fragments. The enzyme in F.hepatica species showed a fragment of 151 bp and a fragment of 312, but in the F.gigantica, three fragments were 151, 93 and 219 bp.36 (73.46%) worms were identified as FASCIOLA GIGANTICA and 13 (26.53%) worms were identified as FASCIOLA HEPATICA. Out of the six infected SHEEP LIVER, 22 were isolated from the Fasciola worms, 13 (59.1%) of which were F.hepatica and 9 (40.9%) of them were F.gigantica. Out of the six infected CATTLE LIVER, 27Fasciola worms were identified, all of which were identified as FASCIOLA GIGANTICA (100%).Conclusion: This study showed that FASCIOLA GIGANTICA is the dominant species in infected LIVERs of the CATTLE in Gorgan city.

Cites

No record.

References

No record.

Cite

APA: Copy

HALAKOU, A., KHAZAN, H., BANDEHPOUR, M., TAGHIPOUR, N., & KAZEMI, B.. (2017). IDENTIFICATION OF FASCIOLA SPECIES BY PCR-RFLP ASSAY IN NORTHERN IRAN. JOURNAL OF GORGAN UNIVERSITY OF MEDICAL SCIENCES, 19(3 (63) ), 99-104. SID. https://sid.ir/paper/79016/en

Vancouver: Copy

HALAKOU A., KHAZAN H., BANDEHPOUR M., TAGHIPOUR N., KAZEMI B.. IDENTIFICATION OF FASCIOLA SPECIES BY PCR-RFLP ASSAY IN NORTHERN IRAN. JOURNAL OF GORGAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2017;19(3 (63) ):99-104. Available from: https://sid.ir/paper/79016/en

IEEE: Copy

A. HALAKOU, H. KHAZAN, M. BANDEHPOUR, N. TAGHIPOUR, and B. KAZEMI, “IDENTIFICATION OF FASCIOLA SPECIES BY PCR-RFLP ASSAY IN NORTHERN IRAN,” JOURNAL OF GORGAN UNIVERSITY OF MEDICAL SCIENCES, vol. 19, no. 3 (63) , pp. 99–104, 2017, [Online]. Available: https://sid.ir/paper/79016/en

Related Journal Papers