DETECTION OF PHOSPHOLIPASE AND TYPE IV PILI GENES IN CLINICAL ISOLATES OF MULTIDRUG-RESISTANT PSEUDOMONAS AERUGINOSA

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MONAZAMI A.; HAGHI F.

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Journal: Journal of Gorgan University of Medical Sciences Year:2017 | Volume:19 | Issue:2 (62) Page(s): 91-97

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Title

DETECTION OF PHOSPHOLIPASE AND TYPE IV PILI GENES IN CLINICAL ISOLATES OF MULTIDRUG-RESISTANT PSEUDOMONAS AERUGINOSA

Author(s)

MONAZAMI A. | HAGHI F. | Issue Writer Certificate

Keywords

EUDOMONAS AERUGINOSAQ2

ANTIBIOTIC RESISTANCEQ2

PHOSPHOLIPASE CQ1

TYPE IV PILIQ1

Abstract

Background and Objective: PsEUDOMONAS AERUGINOSA is an opportunistic pathogen with numerous virulence factors such as phospholipase and TYPE IV PILI. The emergence of multidrug resistant PsEUDOMONAS AERUGINOSAhas become a serious public health threat worldwide. This study was done to determine the frequency of plcH, plcN, pilA and pilB genes in multi-drug resistantPsEUDOMONAS AERUGINOSAisolated from clinical samples.Methods: In this cross-sectional study, 93 isolates of PsEUDOMONAS AERUGINOSA collected from different clinical samples from hospitals of Zanjan, Iran during 2013-14. After identification of isolates by biochemical tests, antibiotic susceptibility testing (Kirby-Bauer) was performed according to CLSI guidelines. Total DNA extracted and PCR was done to detect of plcH, plcN, pilA and pilB genes.Results: Among 93 of PsEUDOMONAS AERUGINOSA isolates, the highest ANTIBIOTIC RESISTANCE related to Erythromycin and Cefoxitin (95.6%) and the lowest resistance related to Amikacin (26.8%).80.6% of isolates were multidrug resistant (MDR). Out of 75 MDR isolates, the frequency of plcH, plcN, pilA and pilB genes was 97.4%, 49.3%, 26.6% and 17.3%, respectively.Conclusion: According to high frequency of PHOSPHOLIPASE C gene (plcH) in MDR PsEUDOMONAS AERUGINOSAisolates which isolated from different clinical samples, presumably this virulence factor plays an important role in pathogenesis of this bacterium.

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APA: Copy

MONAZAMI, A., & HAGHI, F.. (2017). DETECTION OF PHOSPHOLIPASE AND TYPE IV PILI GENES IN CLINICAL ISOLATES OF MULTIDRUG-RESISTANT PSEUDOMONAS AERUGINOSA. JOURNAL OF GORGAN UNIVERSITY OF MEDICAL SCIENCES, 19(2 (62) ), 91-97. SID. https://sid.ir/paper/79370/en

Vancouver: Copy

MONAZAMI A., HAGHI F.. DETECTION OF PHOSPHOLIPASE AND TYPE IV PILI GENES IN CLINICAL ISOLATES OF MULTIDRUG-RESISTANT PSEUDOMONAS AERUGINOSA. JOURNAL OF GORGAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2017;19(2 (62) ):91-97. Available from: https://sid.ir/paper/79370/en

IEEE: Copy

A. MONAZAMI, and F. HAGHI, “DETECTION OF PHOSPHOLIPASE AND TYPE IV PILI GENES IN CLINICAL ISOLATES OF MULTIDRUG-RESISTANT PSEUDOMONAS AERUGINOSA,” JOURNAL OF GORGAN UNIVERSITY OF MEDICAL SCIENCES, vol. 19, no. 2 (62) , pp. 91–97, 2017, [Online]. Available: https://sid.ir/paper/79370/en

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