DETERMINATION OF P53 GENE MUTATIONS IN GASTRIC CANCER BY PCR-SSCP

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JOUSHAGHANI H.R.; KOUCHAKI E.; AMINI P.; DERAKHSHANDEH P.; EHSANI ZONOUZ ABD ALVAHAB; SHABANI M.; KADIVAR M.

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Journal: JOURNAL OF GORGAN UNIVERSITY OF MEDICAL SCIENCES Year:2004 | Volume:5 | Issue:12 Page(s): 36-42

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Information Journal Paper

Title

DETERMINATION OF P53 GENE MUTATIONS IN GASTRIC CANCER BY PCR-SSCP

Author(s)

Keywords

GASTRIC CANCERQ2

IRANQ2

P53 GENEQ2

MUTATIONQ2

PCR-SSCPQ3

Abstract

Background and Objective: GASTRIC CANCER is the second cause of cancer mortality after lung cancer. Approximately 12% of all cancer death are due to GASTRIC CANCER. Tumorgenesis is thought to be a multi step process involving a series of genetic changes in oncogenes and suppressor genes. The most common cancer-~elated genetic change known in human tumors is p53 MUTATION, particularly in GASTRIC CANCER. This sutdy was done to determine P53 GENE MUTATIONs in GASTRIC CANCER. Materials and methods: This study was perfomed on 44 biopsy from patients with GASTRIC CANCER during 2002 in three hospitals in Tehran. For determination ofp53 gene MUTATIONs was performed PCR-SSCP methods Results: The patients group comprised 31 males and 13 females (average age, 60.8 years; ranging from 34 to 84 years). 36 cases (81.8 %) intestinal type, 5 cases (11.4 %) were diffuse type and 3 cases no defined. Forty-four GASTRIC CANCERs of gastric tissues were screened for the mutations of P53 GENE MUTATIONs in Exons 5-8 using the PCR-SSCP analysis. After polyacrylamide gel electrophoresis, nine patients (20.5%) showed an apparent electrophoretic mobility shift between the cancer and other normal samples. One MUTATION in Exon 5 (11.1 %), two were detected in Exon 6 (22.2 %), three were found in Exon 7 (33.3 %), and three were detected in Exon 8 (33.3%). The MUTATION rate was seven of36 (21.2%) in intestinal type and two of (40%) in diffuse type. No significant correlation between P53 GENE MUTATIONs and age and genus was found. Conclusions: This Investigation showed the rate ofp53 gene MUTATION (20.5%) in GASTRIC CANCER in our socitey.

Cites

STUDY OF TWO COMMON P53 GENE MUTATIONS IN GASTRIC CANCER USING PCR-RFLP IN CHAHARMAHAL VA BAKHTIARI PROVINCE, IRAN, 2003

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APA: Copy

JOUSHAGHANI, H.R., KOUCHAKI, E., AMINI, P., DERAKHSHANDEH, P., EHSANI ZONOUZ, ABD ALVAHAB, SHABANI, M., & KADIVAR, M.. (2004). DETERMINATION OF P53 GENE MUTATIONS IN GASTRIC CANCER BY PCR-SSCP. JOURNAL OF GORGAN UNIVERSITY OF MEDICAL SCIENCES, 5(12), 36-42. SID. https://sid.ir/paper/79492/en

Vancouver: Copy

JOUSHAGHANI H.R., KOUCHAKI E., AMINI P., DERAKHSHANDEH P., EHSANI ZONOUZ ABD ALVAHAB, SHABANI M., KADIVAR M.. DETERMINATION OF P53 GENE MUTATIONS IN GASTRIC CANCER BY PCR-SSCP. JOURNAL OF GORGAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2004;5(12):36-42. Available from: https://sid.ir/paper/79492/en

IEEE: Copy

H.R. JOUSHAGHANI, E. KOUCHAKI, P. AMINI, P. DERAKHSHANDEH, ABD ALVAHAB EHSANI ZONOUZ, M. SHABANI, and M. KADIVAR, “DETERMINATION OF P53 GENE MUTATIONS IN GASTRIC CANCER BY PCR-SSCP,” JOURNAL OF GORGAN UNIVERSITY OF MEDICAL SCIENCES, vol. 5, no. 12, pp. 36–42, 2004, [Online]. Available: https://sid.ir/paper/79492/en

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