THE FREQUENCY OF BETA LACTAMASE GENES IN ESCHERICHIA COLI ISOLATES FROM OUTPATIENT SUFFERING FROM URINARY TRACT INFECTIONS IN GUILAN PROVINCE

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

AMIRMOZAFARI N.; BABAIEKASMAIE Z.; MOHSENPOUR M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Yafteh Year:2018 | Volume:19 | Issue:5 (74) Page(s): 43-52

Download Full-Text

Persian Verion

View:

851

Download:

Cites:

Information Journal Paper

Title

THE FREQUENCY OF BETA LACTAMASE GENES IN ESCHERICHIA COLI ISOLATES FROM OUTPATIENT SUFFERING FROM URINARY TRACT INFECTIONS IN GUILAN PROVINCE

Author(s)

AMIRMOZAFARI N. | BABAIEKASMAIE Z. | MOHSENPOUR M. | Issue Writer Certificate

Keywords

ESBLQ2

E. COLIQ2

UTIQ2

Abstract

Background: Escherichia coliis one of the most common causative agent of urinary tract infection. In recent years, resistance to cephalosporins has been considerably on the rise due to production of extended spectrum beta-lactamases (ESBLs). This study was aimed to determine the survey of CTX-M, SHV, TEM, OXA-1, PER-2, and VEB-1 which are the most famous ESBL genes inE. coli isolated from outpatients with UTI in Guilan.Materials and Methods: A total of 2267 urine samples were collected from outpatients suffering from UTIs. After primary biochemical and differential tests like MRVP, Lysin dacarboxilation in LIA medium, Urea hydrolysis test, TSI and Simon citrate test, samples containingE. coli were identified. Antibiotic susceptibilities were determined by disk diffusion. Double disk tests using Cephtriaxon, Amoxiclave and Cephtazidime-clavolonate were performed for phenotypic ESBL production assay. ESBL-producing genes were evaluated by PCR.Results: Among the 2267 urine samples, 167 E. COLI cells were isolated. From these E. COLI isolates, 38.9% were shown to be ESBL producers by the Double disk method. Based on the molecular analysis, the frequency of ESBL-producing genes were, CTX-M (70.32%), TEM (9.64%), SHV (4.88%) OXA-1 (57.2%), and PER-2 (12.1%). VEB-1 was not detected in the analyzed samples. Also, some of the isolates had more than one ESBL-producing gene.Statistically, there was a direct correlation between gender and age with the infection.Conclusion: In this study, more than half of the isolated bacteria were ESBL-producers. As the resistance-inducing genes are carried on mobile genetic elements, rapid detection of resistant species is of major importance to prevent their dissemination.

Cites

No record.

References

No record.

Cite

APA: Copy

AMIRMOZAFARI, N., BABAIEKASMAIE, Z., & MOHSENPOUR, M.. (2018). THE FREQUENCY OF BETA LACTAMASE GENES IN ESCHERICHIA COLI ISOLATES FROM OUTPATIENT SUFFERING FROM URINARY TRACT INFECTIONS IN GUILAN PROVINCE. YAFTEH, 19(5 (74) ), 43-52. SID. https://sid.ir/paper/80122/en

Vancouver: Copy

AMIRMOZAFARI N., BABAIEKASMAIE Z., MOHSENPOUR M.. THE FREQUENCY OF BETA LACTAMASE GENES IN ESCHERICHIA COLI ISOLATES FROM OUTPATIENT SUFFERING FROM URINARY TRACT INFECTIONS IN GUILAN PROVINCE. YAFTEH[Internet]. 2018;19(5 (74) ):43-52. Available from: https://sid.ir/paper/80122/en

IEEE: Copy

N. AMIRMOZAFARI, Z. BABAIEKASMAIE, and M. MOHSENPOUR, “THE FREQUENCY OF BETA LACTAMASE GENES IN ESCHERICHIA COLI ISOLATES FROM OUTPATIENT SUFFERING FROM URINARY TRACT INFECTIONS IN GUILAN PROVINCE,” YAFTEH, vol. 19, no. 5 (74) , pp. 43–52, 2018, [Online]. Available: https://sid.ir/paper/80122/en

Related Journal Papers