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Title

PHENOTYPIC AND MOLECULAR DETECTION OF ESBL PRODUCING CTX-M GENES IN ISOLATED FROM ESCHERICHIA COLI POULTRY WITH COLIBACILLOSIS IN KHORRAMABAD CITY

Pages

  61-70

Abstract

 Background: Most strains of E.COLI with multi drug resistance are major cause of COLIBACILLOSIS in poultry. The economic damages caused byE.coli infections, especially in the poultry industry and also the costs of the disease treatment with antibiotics are very high annually. Therefore, the aim of this study was to investigate the prevalence ofbla CTX-M genes in E.COLI isolates obtained from poultry disease in Khorramabad city.Materials and Methods: In this present study, 100 E.COLI isolates were collected in variable organs of birds with COLIBACILLOSIS and then confirmed with biochemical tests. All the positive samples were there after investigated for the presence ofCTX-M genes by PCR.Results: 4 (%4) out of 100 E.COLI isolates were ESBL positive based on the results of combined disk tests. PCR analysis using the specific primers revealed that %25 contained β-lactamases genes encodingCTX-M.Conclusion: Giving the importance of E.COLI infections in poultry and high prevalence of ESBLproducing strains of rapid diagnostics should used routinely to determine the strains in the laboratories. The results of this study show that theE.coli is saprophytic gastrointestinal reservoir for ESBL. This issue is very important in terms of public health and the transfer of antibiotic resistance to humans.

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    APA: Copy

    RASHIDIAN, E., NADALI, S., NAYEB AGHAEE, S.M., & NORUZIAN, H.. (2018). PHENOTYPIC AND MOLECULAR DETECTION OF ESBL PRODUCING CTX-M GENES IN ISOLATED FROM ESCHERICHIA COLI POULTRY WITH COLIBACILLOSIS IN KHORRAMABAD CITY. YAFTEH, 19(5 (74) ), 61-70. SID. https://sid.ir/paper/80123/en

    Vancouver: Copy

    RASHIDIAN E., NADALI S., NAYEB AGHAEE S.M., NORUZIAN H.. PHENOTYPIC AND MOLECULAR DETECTION OF ESBL PRODUCING CTX-M GENES IN ISOLATED FROM ESCHERICHIA COLI POULTRY WITH COLIBACILLOSIS IN KHORRAMABAD CITY. YAFTEH[Internet]. 2018;19(5 (74) ):61-70. Available from: https://sid.ir/paper/80123/en

    IEEE: Copy

    E. RASHIDIAN, S. NADALI, S.M. NAYEB AGHAEE, and H. NORUZIAN, “PHENOTYPIC AND MOLECULAR DETECTION OF ESBL PRODUCING CTX-M GENES IN ISOLATED FROM ESCHERICHIA COLI POULTRY WITH COLIBACILLOSIS IN KHORRAMABAD CITY,” YAFTEH, vol. 19, no. 5 (74) , pp. 61–70, 2018, [Online]. Available: https://sid.ir/paper/80123/en

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