FETAL SEX DETERMINATION VIA NESTED-PCR METHOD USING AMYLOGENIN GENE

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RAHIMI AM.; SHAH HOSSEINI M.H.; AHANGARI GH.; SHAHSAVAR F.; SEYEDREZAYE TEHRANI SM.

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Journal: Yafteh Year:2004 | Volume:6 | Issue:22 Page(s): 19-26

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Title

FETAL SEX DETERMINATION VIA NESTED-PCR METHOD USING AMYLOGENIN GENE

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Keywords

NESTED- PCRQ2

AMELOGENIN GENEQ4

SEX DETERMINATIONQ3

X-LINKED DISEASEQ4

Abstract

Background: Detection of fetal sex is valuable for parents. The aim of this study was detection of fetal sex in suspect cases recessive related to sex disease in first trimester of pregnancy.Material and method: After collecting C.V samples and separating from mother"s samples, their DNAs have been extracted, then doing PCR, finally the sex of them detected. Also, after increase in sensitivity of system will be able to detection sex of a cell that obtain by PCR. A statistical analysis was made using the fisher test.Results: sex of 74 samples chronic villous detected by PCR only success to relate with many family for following, but also the sex was correct. After increasing in sensitivity of system, detected the sixteen fetuses that was in different stage cellular.Also detected two unfertilized ovum and fertilized ovum but no divided.Conclusion: Sex detection of fetus before delivery, in first trimester of pregnancy will be preventing to new born patient babies, and also decrease the love and relation between mother and her newborn. It can be used in detection of recessive sex related disease in IVF cases for sex detecting and transfer female fetus to her mother. Another application is for control of population. This optimized molecular which designed based on amelogenin target, detect sex in blood samples C.V single cell PCR with high sensitivity & specificity.

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APA: Copy

RAHIMI, AM., SHAH HOSSEINI, M.H., AHANGARI, GH., SHAHSAVAR, F., & SEYEDREZAYE TEHRANI, SM.. (2004). FETAL SEX DETERMINATION VIA NESTED-PCR METHOD USING AMYLOGENIN GENE. YAFTEH, 6(22), 19-26. SID. https://sid.ir/paper/80217/en

Vancouver: Copy

RAHIMI AM., SHAH HOSSEINI M.H., AHANGARI GH., SHAHSAVAR F., SEYEDREZAYE TEHRANI SM.. FETAL SEX DETERMINATION VIA NESTED-PCR METHOD USING AMYLOGENIN GENE. YAFTEH[Internet]. 2004;6(22):19-26. Available from: https://sid.ir/paper/80217/en

IEEE: Copy

AM. RAHIMI, M.H. SHAH HOSSEINI, GH. AHANGARI, F. SHAHSAVAR, and SM. SEYEDREZAYE TEHRANI, “FETAL SEX DETERMINATION VIA NESTED-PCR METHOD USING AMYLOGENIN GENE,” YAFTEH, vol. 6, no. 22, pp. 19–26, 2004, [Online]. Available: https://sid.ir/paper/80217/en

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