STUDY OF GENETIC DIVERSITY OF EXOTIC AND ENDEMIC DAFFODILS (NARCISSUS SPP.) USING RAPD MARKERS

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CHEHRAZI M.; NADERI R.A.; SHAHNEZHAT BOUSHEHRI A.A.; HASANI M.E.

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Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY Year:2008 | Volume:8 | Issue:4 Page(s): 225-236

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Information Journal Paper

Title

STUDY OF GENETIC DIVERSITY OF EXOTIC AND ENDEMIC DAFFODILS (NARCISSUS SPP.) USING RAPD MARKERS

Author(s)

CHEHRAZI M. | NADERI R.A. | SHAHNEZHAT BOUSHEHRI A.A. | HASANI M.E. | Issue Writer Certificate

Keywords

GENETIC DIVERSITYQ2

MOLECULAR MARKERSQ2

NARCISSUS SP.Q3

RAPDQ3

PCRQ3

Abstract

Iranian daffodil, a beautiful and fragrant flower, is one of the oldest ornamental flowers that historically reported before Hegira. The purpose of this study was to evaluate the intra- and inter- population GENETIC DIVERSITY and phylogenetic relationships of Iranian (southern Khorasan, Khuzestan, Kohkiloye and Boyerahmad, Fars, Mazandaran and Golestan provinces) and some exotic daffodils using RAPD markers. Foutteen of 50 random primers showed scorable and significant polymorphism between genotypes. Pair-wise comparisons of unique and shared polymorphic amplification products were used to generate simple matching coefficients. The similarity coefficients were used to construct a dendrogram by Unweighted Pair-Group Method with Arithmetic Averages (UPGMA) using NTSYS-PC. Average similarity coefficients calculated 0.81 ranged from 0.88 (endemics) to 0.48 (exotics). The resulted cluster was subdivided into 9 subgroups. Present results showed variation in exotic genotypes that was more than that of native populations. As wide genetic base is a important issue in plant breeding thus it is necessary to include this in daffodil breeding programs. Among random RAPD primers UBC-96 of 5'-GGCGGCATGG-3' sequence differentiated genotypes into two groups. Therefore, the mentioned primer could be applied for effective preliminary screening of single and double daffodils and saving the time needed for flowering.

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References

AN EVALUATION OF GENETIC DIVERSITY AMONG SOME POMEGRANATE GENOTYPES USING RAPD MARKERS

Cite

APA: Copy

CHEHRAZI, M., NADERI, R.A., SHAHNEZHAT BOUSHEHRI, A.A., & HASANI, M.E.. (2008). STUDY OF GENETIC DIVERSITY OF EXOTIC AND ENDEMIC DAFFODILS (NARCISSUS SPP.) USING RAPD MARKERS. IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY, 8(4), 225-236. SID. https://sid.ir/paper/80804/en

Vancouver: Copy

CHEHRAZI M., NADERI R.A., SHAHNEZHAT BOUSHEHRI A.A., HASANI M.E.. STUDY OF GENETIC DIVERSITY OF EXOTIC AND ENDEMIC DAFFODILS (NARCISSUS SPP.) USING RAPD MARKERS. IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY[Internet]. 2008;8(4):225-236. Available from: https://sid.ir/paper/80804/en

IEEE: Copy

M. CHEHRAZI, R.A. NADERI, A.A. SHAHNEZHAT BOUSHEHRI, and M.E. HASANI, “STUDY OF GENETIC DIVERSITY OF EXOTIC AND ENDEMIC DAFFODILS (NARCISSUS SPP.) USING RAPD MARKERS,” IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY, vol. 8, no. 4, pp. 225–236, 2008, [Online]. Available: https://sid.ir/paper/80804/en

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