DIRECT IN VITRO BULBLET PRODUCTION IN LILIUM LONGIFLORUM CV. ‘GIRONDE’

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MOJTAHEDI N.; KOOBAZ P.; DABIR ASHRAFI O.; HABASHI A.A.

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Paper Information

Journal: IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY Year:2010 | Volume:11 | Issue:3 Page(s): 221-234

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Title

DIRECT IN VITRO BULBLET PRODUCTION IN LILIUM LONGIFLORUM CV. ‘GIRONDE’

Author(s)

MOJTAHEDI N. | KOOBAZ P. | DABIR ASHRAFI O. | HABASHI A.A. | Issue Writer Certificate

Keywords

BAPQ3

BULBLET PRODUCTIONQ3

DIRECT IN VITROQ3

LILIUMQ4

NAAQ4

Abstract

Micropropagtaion of ornamental plants using tissue culture technique, is a new efficient method for propagation of plants. LILIUM is an ornamental plant, which is highly valuable in international flower markets. Due to Iran’s dependency to the importation of LILIUM bulbs, it is highly desirable to propagate this plant efficiently in the country. One of the most effective techniques for propagation of LILIUM is in vitro direct BULBLET PRODUCTION using scale explants. To find the best plant growth regulators concentrations for direct BULBLET PRODUCTION of LILIUM longiflorum cv. ‘Gironde’, four concentrations of BAP (0, 0.03, 0.3, 3 mg l-1) in combination with three concentrations of NAA (0, 0.03, 0.3 mg l-1) were evaluated. Scale explants placed on MS basal medium supplemented with 60 g l-1 sucrose and 7 g l-1 agar after surface sterilization. The substrate for acclimatization and growth of in vitro bulblets were also studied. The experiment conducted in factorial based on completely randomized design (CRD) and statistical analysis was made using SAS software and mean comparison was carried out using Duncan's multiple range test. BULBLET PRODUCTION parameters including number of scales per bulblet, bulblet length, weight and diameter, rooting percentage, and position of bulblet formation in each explant (basal, lateral and distal) were analyzed. The results showed that BAP and NAA had a significant effect on all measured parameters. The combination of 0.03 mg l-1 BAP with 0.3 mg l-1 NAA produced the most bulblet number and the highest rooting percentage. Basal part of the explants, produced the most bulblets in comparison with lateral and distal parts. Peat was the best substrate (soil mixture) for in vitro bulblet's growth and development for LILIUM longiflorum cv. ‘Gironde’.

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References

EFFECTS OF PLANT GROWTH REGULATORS NAA, BAP, DIFFERENT EXPLANTS SCALE AND PHOTOPERIOD ON TISSUE CULTURE OF LILIUM LEDEBOURII BOISS

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APA: Copy

MOJTAHEDI, N., KOOBAZ, P., DABIR ASHRAFI, O., & HABASHI, A.A.. (2010). DIRECT IN VITRO BULBLET PRODUCTION IN LILIUM LONGIFLORUM CV. ‘GIRONDE’. IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY, 11(3), 221-234. SID. https://sid.ir/paper/80822/en

Vancouver: Copy

MOJTAHEDI N., KOOBAZ P., DABIR ASHRAFI O., HABASHI A.A.. DIRECT IN VITRO BULBLET PRODUCTION IN LILIUM LONGIFLORUM CV. ‘GIRONDE’. IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY[Internet]. 2010;11(3):221-234. Available from: https://sid.ir/paper/80822/en

IEEE: Copy

N. MOJTAHEDI, P. KOOBAZ, O. DABIR ASHRAFI, and A.A. HABASHI, “DIRECT IN VITRO BULBLET PRODUCTION IN LILIUM LONGIFLORUM CV. ‘GIRONDE’,” IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY, vol. 11, no. 3, pp. 221–234, 2010, [Online]. Available: https://sid.ir/paper/80822/en

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