PHYLOGENETIC RELATIONSHIPS AMONG SOME IRANIAN POMEGRANATE ACCESSIONS REVEALED BY INTER-SIMPLE SEQUENCE REPEAT (ISSR) MARKERS

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GHOBADI S.; KHOUSHKHOUI MORTEZA; TABATABAEI B.E.S.

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Journal: IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY Year:2005 | Volume:6 | Issue:3 Page(s): 111-120

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Title

PHYLOGENETIC RELATIONSHIPS AMONG SOME IRANIAN POMEGRANATE ACCESSIONS REVEALED BY INTER-SIMPLE SEQUENCE REPEAT (ISSR) MARKERS

Author(s)

GHOBADI S. | KHOUSHKHOUI MORTEZA | TABATABAEI B.E.S. | Issue Writer Certificate

Keywords

DNA MARKERSQ2

GENETIC DIVERSITYQ4

ISSRQ4

POLYMORPHISMQ4

POMEGRANATEQ4

Abstract

ISSR-PCR markers were analyzed to study phylogenetic relationships among 24 accessions of Iranian POMEGRANATEs (Punica granatum L.). DNA from young and healthy leaves of each accession were extracted and used for the analysis and in PCR reactions. A total of six primers and their combinations including BC825, BC844, BC850, BC825× BC844, BC825×BC850 and LK7 were used in PCR reactions. A dendrogram based on the unweighed pair-group method, arithmetic average cluster analysis was constructed based on a similarity matrix derived from 64 polymorphic ISSR fragments generated by the primers. At 65% similarity levels the accessions classified into five groups (19 accessions in one independent group and the rest in 4 groups). Although the POMEGRANATE accessions under investigation were divergent in morphology, the genetic distances among them were relatively small. The comparison of the results obtained in this study with RAPDs indicated that ISSR markers gave a better band reproducibility and high efficiency in grouping accessions. Relationships among these POMEGRANATE accessions revealed by ISSR markers were not generally in agreement with previous taxonomic classifications with RAPD markers. This indicates that separation of POMEGRANATE only on the basis of morphological characters is not sufficient, and using of molecular methods is necessary.

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APA: Copy

GHOBADI, S., KHOUSHKHOUI, MORTEZA, & TABATABAEI, B.E.S.. (2005). PHYLOGENETIC RELATIONSHIPS AMONG SOME IRANIAN POMEGRANATE ACCESSIONS REVEALED BY INTER-SIMPLE SEQUENCE REPEAT (ISSR) MARKERS. IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY, 6(3), 111-120. SID. https://sid.ir/paper/80879/en

Vancouver: Copy

GHOBADI S., KHOUSHKHOUI MORTEZA, TABATABAEI B.E.S.. PHYLOGENETIC RELATIONSHIPS AMONG SOME IRANIAN POMEGRANATE ACCESSIONS REVEALED BY INTER-SIMPLE SEQUENCE REPEAT (ISSR) MARKERS. IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY[Internet]. 2005;6(3):111-120. Available from: https://sid.ir/paper/80879/en

IEEE: Copy

S. GHOBADI, MORTEZA KHOUSHKHOUI, and B.E.S. TABATABAEI, “PHYLOGENETIC RELATIONSHIPS AMONG SOME IRANIAN POMEGRANATE ACCESSIONS REVEALED BY INTER-SIMPLE SEQUENCE REPEAT (ISSR) MARKERS,” IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY, vol. 6, no. 3, pp. 111–120, 2005, [Online]. Available: https://sid.ir/paper/80879/en

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