EFFECTS OF EXPLANTS, MEDIA AND GROWTH REGULATORS ON MICROPROPAGATION OF SWEET LIME (CITRUS LIMETTA SWING.)

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KHOUSHKHOUI MORTEZA; REZAZADEH R.

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Journal: IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY Year:2000 | Volume:1 | Issue:1-2 Page(s): 1-14

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Title

EFFECTS OF EXPLANTS, MEDIA AND GROWTH REGULATORS ON MICROPROPAGATION OF SWEET LIME (CITRUS LIMETTA SWING.)

Author(s)

KHOUSHKHOUI MORTEZA | REZAZADEH R. | Issue Writer Certificate

Keywords

CITRUS LIMETTA SWINGQ3

MICROPROPAGATIONQ3

SWEET LIMEQ3

Abstract

Experiments were conducted to micropropagate SWEET LIME from shoot- tip (5 mm long) and single-node (5-10 mm long) explants. The explants were surface sterilized and cultured on basic MS medium supplement with various concentrations of benzyladenin (BA) in combination with different concentrations of kinetin (Kin) and naphthaleneacetic acid (NAA). A combination of 1.25 mg I-1 BA with 0.5 mg I-1, NAA and 0.5 mg I-1 Kin was the most suitable, treatment for shoot proliferation using both explants. Shoots obtained from in vitro culture were subcultured four times, each with a 4-week interval, on a fresh medium. Effects of some other variables, such as explant source and adding gibberellic acid (GA3) to the medium, on shoot proliferation rate, were also studied. Single-node, explants, compared to shoot-, tip explants had a better response to in vitro conditions. For rooting, basic MS medium with half or full concentrations of salts plus vitamins, 1.5 or 3%, sucrose and 6 g I-1 agar were used. A five-second quick-dip in sterilized 500 mg I-1 NAA solution before inserting the explants on the medium or direct transferring of the explants on a medium containing 0.25 mg I-1 BA and 0.25 mg I-1 NAA resulted to a good rooting after 4 weeks. The rooted plantlets were successfully acclimatized in a sterilized vermiculite mixture which was superior to quartz, peat moss or mixtures of the three rooting media. Plantlets irrigated with one-eight-concentration of MS salt solution in first week, were easily acclimatized.

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APA: Copy

KHOUSHKHOUI, MORTEZA, & REZAZADEH, R.. (2000). EFFECTS OF EXPLANTS, MEDIA AND GROWTH REGULATORS ON MICROPROPAGATION OF SWEET LIME (CITRUS LIMETTA SWING.). IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY, 1(1-2), 1-14. SID. https://sid.ir/paper/80899/en

Vancouver: Copy

KHOUSHKHOUI MORTEZA, REZAZADEH R.. EFFECTS OF EXPLANTS, MEDIA AND GROWTH REGULATORS ON MICROPROPAGATION OF SWEET LIME (CITRUS LIMETTA SWING.). IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY[Internet]. 2000;1(1-2):1-14. Available from: https://sid.ir/paper/80899/en

IEEE: Copy

MORTEZA KHOUSHKHOUI, and R. REZAZADEH, “EFFECTS OF EXPLANTS, MEDIA AND GROWTH REGULATORS ON MICROPROPAGATION OF SWEET LIME (CITRUS LIMETTA SWING.),” IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY, vol. 1, no. 1-2, pp. 1–14, 2000, [Online]. Available: https://sid.ir/paper/80899/en

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