EFFECTS OF MEDIA AND GROWTH REGULATORS ON GARDEN THYME (THYMUS VULGARIS L.) MICROPROPAGATION

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HOSSEINI BEHESHTI B.; KHOUSHKHOUI MORTEZA

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Paper Information

Journal: IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY Year:2005 | Volume:6 | Issue:2 Page(s): 61-68

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Information Journal Paper

Title

EFFECTS OF MEDIA AND GROWTH REGULATORS ON GARDEN THYME (THYMUS VULGARIS L.) MICROPROPAGATION

Author(s)

HOSSEINI BEHESHTI B. | KHOUSHKHOUI MORTEZA | Issue Writer Certificate

Keywords

IN VITRO CULTUREQ3

MEDICINAL PLANTSQ3

MICROPROPAGATEQ2

THYMEQ2

THYMUSQ2

TISSUE CULTUREQ2

Abstract

Experiments were conducted to MICROPROPAGATE the garden THYME (Thymus vulgaris L.) using stem single-node explants. Ten mm long stem explants were disinfested by 10% liquid bleach for 10 min and then rinsed three times with sterilized distilled water. Fun and half-strength Murashige and Skoog (MS) media were compared. Both media were supplemented with benzyladenine (BA) and α-naphthaleneacetic acid (NAA) at different concentrations. The best proliferation rate was obtained using medium containing 1 mg 1-1 BA and 0.2 mg 1-1 NAA. In a separate experiment, the explants were four times sub cultured on a medium containing best growth regulator concentrations of establishment stage with a 4-week interval. No significant differences were found between subcultures. For rooting, shoots were placed on MS medium supplemented with different concentrations of growth regulators, 25 g 1-1 sucrose and 8 g 1-1 agar. After 8 wk the best rooting treatment was application of 1.25 mg 1-1 NAA without BA. Rooted plantlets were transferred to pasteurized media consisting a mixture of 1/6 peat, 1/6 sand, 1/6 loam soil and 3/6 perlite. Plantlets were gradually acclimatized to natural conditions after 2 weeks.

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APA: Copy

HOSSEINI BEHESHTI, B., & KHOUSHKHOUI, MORTEZA. (2005). EFFECTS OF MEDIA AND GROWTH REGULATORS ON GARDEN THYME (THYMUS VULGARIS L.) MICROPROPAGATION. IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY, 6(2), 61-68. SID. https://sid.ir/paper/80916/en

Vancouver: Copy

HOSSEINI BEHESHTI B., KHOUSHKHOUI MORTEZA. EFFECTS OF MEDIA AND GROWTH REGULATORS ON GARDEN THYME (THYMUS VULGARIS L.) MICROPROPAGATION. IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY[Internet]. 2005;6(2):61-68. Available from: https://sid.ir/paper/80916/en

IEEE: Copy

B. HOSSEINI BEHESHTI, and MORTEZA KHOUSHKHOUI, “EFFECTS OF MEDIA AND GROWTH REGULATORS ON GARDEN THYME (THYMUS VULGARIS L.) MICROPROPAGATION,” IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY, vol. 6, no. 2, pp. 61–68, 2005, [Online]. Available: https://sid.ir/paper/80916/en

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