A STUDY OF THE STABILITY OF ESCHERICHIA COLI ON DNA BANKING CARD (DBC) FOR MOLECULAR TESTS

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SHAHAB MOVAHHED ZAHRA; ZEINALI SIROUS; ASLANI MOHAMMAD MEHDI

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Journal: Pathobiology Research Year:2012 | Volume:14 | Issue:4 Page(s): 96-103

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Information Journal Paper

Title

A STUDY OF THE STABILITY OF ESCHERICHIA COLI ON DNA BANKING CARD (DBC) FOR MOLECULAR TESTS

Author(s)

SHAHAB MOVAHHED ZAHRA | ZEINALI SIROUS | ASLANI MOHAMMAD MEHDI | Issue Writer Certificate

Keywords

DNA BANKING CARD (DBC)Q2

BACTERIA SUSPENSIONQ2

STORING METHOD OF BACTERIAL DNAQ2

REAL-TIME PCRQ2

Abstract

Objective: Traditional methods for storing microbial DNA samples use the low-temperatures of 4oC, -20oC or -80oC. The DBC Card is a new method of storing microbial DNA at room temperature. This study evaluates the stability and determine the best STORING METHOD OF BACTERIAL DNA on the DBC card.Methods: In this study, we used a pair of primers from the conserved domain of 16srRNA designed to identify Escherichia coli. Four different types of samples we prepared: i) bacterial suspension, ii) bacterial DNA extracted by the phenol-chloroform method, iii) bacterial lysate with lysis buffer and iv) bacterial DNA produced by the boiling method. All four samples were spotted on separate DBC cards and dried at room temperature. After periods of 3, 5 and 7 months, Escherichia coli samples were checked for DNA stability with two molecular techniques, conventional PCR with 1, 2 and 3 disks as a source of bacterial DNA (1 mm diameter) and REAL-TIME PCR.Results: Data showed that DNA stability was maintained after 7 months on a DBC disk, even using only one disk as a DNA source. The bacterial suspension was the best method for long-term storage of Escherichia coli DNA on the DBC Card.Conclusion: In traditional methods for storing sample, DNA quality reduces after freezing and thawing. However in the DBC method, DNA quality was maintained for a long duration. Advantages of the DBC method are easy sample handing, low cost, faster extraction, and reduced individual and environmental contamination.

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APA: Copy

SHAHAB MOVAHHED, ZAHRA, ZEINALI, SIROUS, & ASLANI, MOHAMMAD MEHDI. (2012). A STUDY OF THE STABILITY OF ESCHERICHIA COLI ON DNA BANKING CARD (DBC) FOR MOLECULAR TESTS. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), 14(4), 96-103. SID. https://sid.ir/paper/80951/en

Vancouver: Copy

SHAHAB MOVAHHED ZAHRA, ZEINALI SIROUS, ASLANI MOHAMMAD MEHDI. A STUDY OF THE STABILITY OF ESCHERICHIA COLI ON DNA BANKING CARD (DBC) FOR MOLECULAR TESTS. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES)[Internet]. 2012;14(4):96-103. Available from: https://sid.ir/paper/80951/en

IEEE: Copy

ZAHRA SHAHAB MOVAHHED, SIROUS ZEINALI, and MOHAMMAD MEHDI ASLANI, “A STUDY OF THE STABILITY OF ESCHERICHIA COLI ON DNA BANKING CARD (DBC) FOR MOLECULAR TESTS,” PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), vol. 14, no. 4, pp. 96–103, 2012, [Online]. Available: https://sid.ir/paper/80951/en

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