DETECTION OF NHE COMPLEX GENES IN BACILLUS CEREUS ISOLATED FROM RICE SAMPLES FROM ZANJAN, IRAN BY MULTIPLEX PCR

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DEILAMI KHIABANI ZAHRA; NOORI ENAYAT; RAHNAMA MEHDI; SHAPOURI REZA; BIGDELO YADOLLAH; GHAMARI DENA; ASEFI HAVVA

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Journal: Pathobiology Research Year:2012 | Volume:14 | Issue:4 Page(s): 88-94

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Title

DETECTION OF NHE COMPLEX GENES IN BACILLUS CEREUS ISOLATED FROM RICE SAMPLES FROM ZANJAN, IRAN BY MULTIPLEX PCR

Author(s)

Keywords

BACILLUS CEREUSQ2

RICEQ2

NHE COMPLEXQ2

MULTIPLEX PCRQ2

Abstract

Objective: BACILLUS CEREUS (B. cereus) is a gram-positive, spore-forming bacteria widely distributed in the environment. This bacteria is an opportunistic human pathogen that can cause diarrheal and emetic types of food poisoning. The diarrhea type of food poisoning can be caused by hemolysin BL (HBL), non-hemolytic (NHE), and cytotoxin K enterotoxins. RICE is commonly contaminated with B. cereus. The objective of this study is to detect enterotoxigenic genes of the NHE COMPLEX and assess their incidence in B. cereus isolates in RICE samples from Zanjan, Iran.Methods: We randomly purchased 10 different RICE samples from food stores and cultured them in PEMPA. Following biochemical testing, the bacterial colonies were identified by PCR. B. cereus isolates were checked for the NHE COMPLEX genes by specific primers using MULTIPLEX PCR.Results: Results showed that RICE samples were contaminated with B. cereus. The NHE COMPLEX genes were found in 8 bacterial samples.Conclusion: B. cereus is able to tolerate high temperatures; in cooked RICE the spores can undergo germination by reheating. The results of this study have shown that NHE MULTIPLEX PCR is a prompt, reliable method for the differentiation between non-enterotoxigenic and enterotoxigenic isolates of B. cereus. Despite its common dietary role, RICE in Iran has rarely been investigated from a microbiological point of view. Enhancing awareness about virulence and prevalence of genes involved in food poisoning would be effective in the prevention of food poisoning.

Cites

TO DETERMINE THE FREQUENCY OF BACILLUS CEREUS IN POWDERED MILK INFANT FORMULA CONSUMING IN NEONATAL INTENSIVE CARE UNIT (NICU) IN TEHRAN HOSPITALS IN 2013-14

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APA: Copy

DEILAMI KHIABANI, ZAHRA, NOORI, ENAYAT, RAHNAMA, MEHDI, SHAPOURI, REZA, BIGDELO, YADOLLAH, GHAMARI, DENA, & ASEFI, HAVVA. (2012). DETECTION OF NHE COMPLEX GENES IN BACILLUS CEREUS ISOLATED FROM RICE SAMPLES FROM ZANJAN, IRAN BY MULTIPLEX PCR. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), 14(4), 88-94. SID. https://sid.ir/paper/80952/en

Vancouver: Copy

DEILAMI KHIABANI ZAHRA, NOORI ENAYAT, RAHNAMA MEHDI, SHAPOURI REZA, BIGDELO YADOLLAH, GHAMARI DENA, ASEFI HAVVA. DETECTION OF NHE COMPLEX GENES IN BACILLUS CEREUS ISOLATED FROM RICE SAMPLES FROM ZANJAN, IRAN BY MULTIPLEX PCR. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES)[Internet]. 2012;14(4):88-94. Available from: https://sid.ir/paper/80952/en

IEEE: Copy

ZAHRA DEILAMI KHIABANI, ENAYAT NOORI, MEHDI RAHNAMA, REZA SHAPOURI, YADOLLAH BIGDELO, DENA GHAMARI, and HAVVA ASEFI, “DETECTION OF NHE COMPLEX GENES IN BACILLUS CEREUS ISOLATED FROM RICE SAMPLES FROM ZANJAN, IRAN BY MULTIPLEX PCR,” PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), vol. 14, no. 4, pp. 88–94, 2012, [Online]. Available: https://sid.ir/paper/80952/en

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