THE EFFECT OF BLOOD STORAGE ON APOPTOSIS INDUCTION OF JURKAT CELLS: AN IN VITRO MODEL

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

HAJHASHEMI M.; POURFATH ELAH A.A.; AGHAEIPOUR M.; MOAZENI S.M.; NIKOU GOFTAR M.; ZARE AHAD

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Pathobiology Research Year:2009 | Volume:12 | Issue:1 Page(s): 25-32

Download Full-Text

Persian Verion

View:

718

Download:

Cites:

Information Journal Paper

Title

THE EFFECT OF BLOOD STORAGE ON APOPTOSIS INDUCTION OF JURKAT CELLS: AN IN VITRO MODEL

Author(s)

Keywords

APOPTOSISQ2

TRIMQ3

ANNEXIN VQ3

JURKAT CELLQ3

Abstract

Objective: It is a firm belief that blood transfusion is life-saving in many situations, but at the same time transfusion complications could be life-threatening.The possible effects of blood Transfusion Related Immunomodulatory (TRIM) and its related mechanisms are one of the important debatable subjects in the field of blood transfusion medicine. One of the mechanisms through which transfusion can induced TRIM effects in recipient is APOPTOSIS induction. Aim of this study was to investigate the apoptotic effects of stored blood in an in vitro model.Materials and Methods: To evaluate the apoptotic effects of blood storage, we studied the effect of the plasma (from whole blood) during storage on days 3, 10, 21 and 35 on JURKAT CELLs, which are sensitive to APOPTOSIS .The plasma of whole blood was separated by centrifugation on different days. Then, JURKAT CELLs were cultured with plasma for 24 hours. Finally APOPTOSIS level was studied by using flowcytometry for analyzing ANNEXIN V on JURKAT CELLs (by SeroTec ANNEXIN V: FITC Assay Kit).Results: The percentages of APOPTOSIS on days 3, 10, 21 and 35 were 3.85±1.52, 5.27±2.12, 8.44±1.90, 12.01±2.32, respectively. The percentages of apoptotic cells in negative and positive control group was 3.85±1.94 and 65.80±2.28, respectively.Conclusion: The results of this study showed that plasma of whole blood have the apoptotic effects which enhanced during the storage of plasma. This in vitro model is also suitable for studying other TRIM related mechanisms.

Cites

No record.

References

No record.

Cite

APA: Copy

HAJHASHEMI, M., POURFATH ELAH, A.A., AGHAEIPOUR, M., MOAZENI, S.M., NIKOU GOFTAR, M., & ZARE, AHAD. (2009). THE EFFECT OF BLOOD STORAGE ON APOPTOSIS INDUCTION OF JURKAT CELLS: AN IN VITRO MODEL. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), 12(1), 25-32. SID. https://sid.ir/paper/80972/en

Vancouver: Copy

HAJHASHEMI M., POURFATH ELAH A.A., AGHAEIPOUR M., MOAZENI S.M., NIKOU GOFTAR M., ZARE AHAD. THE EFFECT OF BLOOD STORAGE ON APOPTOSIS INDUCTION OF JURKAT CELLS: AN IN VITRO MODEL. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES)[Internet]. 2009;12(1):25-32. Available from: https://sid.ir/paper/80972/en

IEEE: Copy

M. HAJHASHEMI, A.A. POURFATH ELAH, M. AGHAEIPOUR, S.M. MOAZENI, M. NIKOU GOFTAR, and AHAD ZARE, “THE EFFECT OF BLOOD STORAGE ON APOPTOSIS INDUCTION OF JURKAT CELLS: AN IN VITRO MODEL,” PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), vol. 12, no. 1, pp. 25–32, 2009, [Online]. Available: https://sid.ir/paper/80972/en

Related Journal Papers