THE STUDY OF CELL VIABILITY AND ALKALINE PHOSPHATASE ACTIVITY OF SAOS-2 CELLS CONTACT WITH OSTEON AND CERASOEB IN VITRO

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AYOUBIAN NADER; FOROUTAN TAHEREH; JAMSHIDIFAR AZIN; MOSAFFA NARIMAN

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Journal: Pathobiology Research Year:2011 | Volume:13 | Issue:4 Page(s): 13-19

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Title

THE STUDY OF CELL VIABILITY AND ALKALINE PHOSPHATASE ACTIVITY OF SAOS-2 CELLS CONTACT WITH OSTEON AND CERASOEB IN VITRO

Author(s)

AYOUBIAN NADER | FOROUTAN TAHEREH | JAMSHIDIFAR AZIN | MOSAFFA NARIMAN | Issue Writer Certificate

Keywords

BONE SUBSTITUTE REPLACEMENTQ3

CELL VIABILITYQ3

CELL LINE SAOS-2Q3

MORPHOLOGYQ3

Abstract

Objective: Osteon has been introduced as a bone substitute material. It is biphasic calcium phosphate (BCP) that use in dentistry. The aim of the present study was to evaluate the effect of osteon on the proliferation, CELL VIABILITY and differentiation of saos-2 cells in vitro. Also it was compared with cerasorb bone graft.Materials and Methods: Two different bone grafts materials, osteon and cerasorb, were used to evaluate the effect on proliferation and differentiation rate of saos-2 cells. On day 15 the cell proliferation and CELL VIABILITY was measured by MTT assay. For determination of differentiation, alkaline phosphatase and alizarin red test was used.Results: Osteon and cerasorb groups showed significantly higher alkaline phosphatase activity and cell differentiation. CELL VIABILITY of both bone grafts was significantly lower than control group and cell proliferation was higher in osteon group. Osteon has more suitable biological property compare to cerasorb.Conclusions: The results of the present study showed that osteon and cersorb bone grafts allow proliferation and differentiation of saos-2 cells in vitro.

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APA: Copy

AYOUBIAN, NADER, FOROUTAN, TAHEREH, JAMSHIDIFAR, AZIN, & MOSAFFA, NARIMAN. (2011). THE STUDY OF CELL VIABILITY AND ALKALINE PHOSPHATASE ACTIVITY OF SAOS-2 CELLS CONTACT WITH OSTEON AND CERASOEB IN VITRO. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), 13(4), 13-19. SID. https://sid.ir/paper/81026/en

Vancouver: Copy

AYOUBIAN NADER, FOROUTAN TAHEREH, JAMSHIDIFAR AZIN, MOSAFFA NARIMAN. THE STUDY OF CELL VIABILITY AND ALKALINE PHOSPHATASE ACTIVITY OF SAOS-2 CELLS CONTACT WITH OSTEON AND CERASOEB IN VITRO. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES)[Internet]. 2011;13(4):13-19. Available from: https://sid.ir/paper/81026/en

IEEE: Copy

NADER AYOUBIAN, TAHEREH FOROUTAN, AZIN JAMSHIDIFAR, and NARIMAN MOSAFFA, “THE STUDY OF CELL VIABILITY AND ALKALINE PHOSPHATASE ACTIVITY OF SAOS-2 CELLS CONTACT WITH OSTEON AND CERASOEB IN VITRO,” PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), vol. 13, no. 4, pp. 13–19, 2011, [Online]. Available: https://sid.ir/paper/81026/en

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