CLONING AND SEQUENCING OF GRANULAR ANTIGEN7 (GRA7) IN TOPO VECTOR AND TRANSFORMATION IN TOP10

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GHAFFARIFAR FATEMEH; NOURDIN R.; DALIMI A.A.H.; ROUDBAR MOHAMMADI SH.; GHASEMI NIKOU S.; SHARIFI ZOHREH

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Journal: Pathobiology Research Year:2009 | Volume:11 | Issue:3-4 Page(s): 73-80

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Title

CLONING AND SEQUENCING OF GRANULAR ANTIGEN7 (GRA7) IN TOPO VECTOR AND TRANSFORMATION IN TOP10

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Keywords

TOXOPLASMA GONDIIQ3

GRA7 GENEQ3

CLONINGQ3

SEQUENCINGQ3

Abstract

Objective: Toxoplasmosis, caused by an intracellular protozoan parasite, and the TOXOPLASMA GONDII, is widespread throughout the world. In recent years, significant progress has been made in the identification of vaccine candidates which could induce a protective response. GRA7, an excretory 29 kDa TOXOPLASMA GONDII a dense granular antigen released by infected host cells. In tachyzoite-infected cells, p29 accumulates within the parasitophorous vacuole and co-localizes with its delimiting membrane. Materials and Methods: In the present work, first genomic DNA of TOXOPLASMA GONDII was extracted and used for amplifying of GRA7 GENE as a template. Then PCR product was extracted from agarose gel and cloned into TOPO vector. The plasmid containing GRA7 GENE was extracted from the transformed bacteria (TOP10 strain) and sequenced. Results: Sequence analysis of GRA7 GENE cloned into TOPO vector showed only one base difference when composed with the gene bank sequence for RH strain was only one base. Conclusion: The results indicated that this clone is suitable for subcloning in Prokaryotic and Eukaryotic plasmid.

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APA: Copy

GHAFFARIFAR, FATEMEH, NOURDIN, R., DALIMI, A.A.H., ROUDBAR MOHAMMADI, SH., GHASEMI NIKOU, S., & SHARIFI, ZOHREH. (2009). CLONING AND SEQUENCING OF GRANULAR ANTIGEN7 (GRA7) IN TOPO VECTOR AND TRANSFORMATION IN TOP10. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), 11(3-4), 73-80. SID. https://sid.ir/paper/81081/en

Vancouver: Copy

GHAFFARIFAR FATEMEH, NOURDIN R., DALIMI A.A.H., ROUDBAR MOHAMMADI SH., GHASEMI NIKOU S., SHARIFI ZOHREH. CLONING AND SEQUENCING OF GRANULAR ANTIGEN7 (GRA7) IN TOPO VECTOR AND TRANSFORMATION IN TOP10. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES)[Internet]. 2009;11(3-4):73-80. Available from: https://sid.ir/paper/81081/en

IEEE: Copy

FATEMEH GHAFFARIFAR, R. NOURDIN, A.A.H. DALIMI, SH. ROUDBAR MOHAMMADI, S. GHASEMI NIKOU, and ZOHREH SHARIFI, “CLONING AND SEQUENCING OF GRANULAR ANTIGEN7 (GRA7) IN TOPO VECTOR AND TRANSFORMATION IN TOP10,” PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), vol. 11, no. 3-4, pp. 73–80, 2009, [Online]. Available: https://sid.ir/paper/81081/en

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