DENDROSOMAL CURCUMIN INDUCED APOPTOSIS BY SUPPRESSION OF PLURIPOTENCY GENES IN 5637 BLADDER CANCER CELLS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

TAHMASEBI MIRGANI MARYAM; SADEGHIZADEH MAJID; NAJAFI FARHOOD; MOWLA SEYED JAVAD

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Pathobiology Research Year:2013 | Volume:16 | Issue:1 Page(s): 23-39

Download Full-Text

Persian Verion

View:

1,045

Download:

Cites:

Information Journal Paper

Title

DENDROSOMAL CURCUMIN INDUCED APOPTOSIS BY SUPPRESSION OF PLURIPOTENCY GENES IN 5637 BLADDER CANCER CELLS

Author(s)

TAHMASEBI MIRGANI MARYAM | SADEGHIZADEH MAJID | NAJAFI FARHOOD | MOWLA SEYED JAVAD | Issue Writer Certificate

Keywords

CURCUMINQ2

DENDROSOMEQ2

CELL-PROGRAMMED DEATHQ2

BLADDER CANCERQ3

PLURIPOTENCY GENESQ2

Abstract

Objective: The anti-cancer properties of CURCUMIN, a poliphenol extract from the rhizome of curry, has been confirmed by many investigators. However, low levels of uptake, tissue distribution and rapid metabolism has limited its application as an anti-cancer drug. This study is aimed at increasing CURCUMIN's water solubility due to a biodegradable, neutral and non-toxic micellar nano-carrier called DENDROSOME. This study intends to evaluate the role of dendrosomal-CURCUMIN (DNC) in BLADDER CANCER cell growth.Methods: We performed the MTT assay, flow cytometry and Annexin V-FLUOS (as an apoptosis detection kit) to evaluate cell death. The genetic mechanism of DNC-induced apoptosis was accomplished by a study of the relative expressions of OCT4A, OCT4B1, SOX-2 and Nanog using real-time PCR.Results: DNC-induced cell death complied with a time and dose-dependent paradigm in the 5637 cell line. Cell cycle analysis revealed that the number of cells increased in pre- G1 and gradually decreased in G1 and S phases. This showed the inhibitory property of dendrosomal-CURCUMIN on DNA synthesis. Data from real-time PCR determined that expressions of OCT4A, OCT4B1, SOX-2 and Nanog could be related to 5637 cancer cell growth. Dendrosomal-CURCUMIN significantly suppressed mRNA expression of the above mentioned genes (p<0.01).Conclusion: The data showed that DNC induced apoptosis by suppression of PLURIPOTENCY GENES in 5637 BLADDER CANCER cells, which confirmed the useful characteristic of nano-drug in BLADDER CANCER therapy.

Cites

References

No record.

Cite

APA: Copy

TAHMASEBI MIRGANI, MARYAM, SADEGHIZADEH, MAJID, NAJAFI, FARHOOD, & MOWLA, SEYED JAVAD. (2013). DENDROSOMAL CURCUMIN INDUCED APOPTOSIS BY SUPPRESSION OF PLURIPOTENCY GENES IN 5637 BLADDER CANCER CELLS. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), 16(1), 23-39. SID. https://sid.ir/paper/81175/en

Vancouver: Copy

TAHMASEBI MIRGANI MARYAM, SADEGHIZADEH MAJID, NAJAFI FARHOOD, MOWLA SEYED JAVAD. DENDROSOMAL CURCUMIN INDUCED APOPTOSIS BY SUPPRESSION OF PLURIPOTENCY GENES IN 5637 BLADDER CANCER CELLS. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES)[Internet]. 2013;16(1):23-39. Available from: https://sid.ir/paper/81175/en

IEEE: Copy

MARYAM TAHMASEBI MIRGANI, MAJID SADEGHIZADEH, FARHOOD NAJAFI, and SEYED JAVAD MOWLA, “DENDROSOMAL CURCUMIN INDUCED APOPTOSIS BY SUPPRESSION OF PLURIPOTENCY GENES IN 5637 BLADDER CANCER CELLS,” PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), vol. 16, no. 1, pp. 23–39, 2013, [Online]. Available: https://sid.ir/paper/81175/en

Related Journal Papers