3D CULTURE OF UMBLICAL CORD BLOOD-DRIVED
CD34+ CELLS ON A DBM SCAFFOLD COATED BY CORD BLOOD–DERIVED UNRESTRICTED SOMATIC STEM CELLS (USSC)

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VAZIFEH SHIRAN N.; SOLEYMANI MASOUD; MORTAZAVI YOUSEF; KAVIANI S.; ABOU ALGHASEMI H.; NIKOU GOFTAR M.

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Journal: Pathobiology Research Year:2010 | Volume:13 | Issue:3 Page(s): 63-81

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Title

3D CULTURE OF UMBLICAL CORD BLOOD-DRIVED CD34+ CELLS ON A DBM SCAFFOLD COATED BY CORD BLOOD–DERIVED UNRESTRICTED SOMATIC STEM CELLS (USSC)

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Keywords

UCB-CD34+ CELLSQ3

EXPANSIONQ3

USSCQ3

3D SCAFFOLDQ2

DBMQ2

Abstract

Objective: Allogeneic transplantation with umbilical cord blood (UCB) in adult recipients is limited mainly by a low CD34+ cell dose. To overcome this shortcoming, human placenta as a novel source of human mesenchymal progenitor cell (MPC)- unrestricted somatic stem cells (USSC)- was incorporated in an attempt to expand CD34+ cells from UCB. To provide a similar environment in vitro, we coated DBM scaffold with USSC cells as the matrix for support UCB-CD34+ cells growth.Materials and Methods: Human placenta USSC was isolated and characterized by morphologic and immunophenotypical analysis. UCB CD34+ cells were expanded by coculture with placental USSC in 2D and 3D environment. Suitable aliquots of cells were used to monitor cell production, clonogenic activity, and long-term culture-initiating culture (LTC-IC) output.Results: Ex vivo EXPANSION of UCB hematopoietic cells, when cultured in different 2D conditions and 3D condition for 3 weeks, was significantly enhanced, the total cell count increased within the 28-day period. For total CFC, the highest CFC EXPANSION was observed at day 14. Flow cytometry analysis of the percentage of CD34+ cells showed a decline in USSC cocultures in 2D and 3D condition at 3 weeks.Conclusion: These results strongly suggest that human USSC may be a suitable feeder layer for EXPANSION of hematopoietic progenitors from UCB in vitro and USSC- coated DBM can therefore provide an ex vivo mimicry of bone marrow by enhancing of surface/ volume ratio and feeder layers, recapitulate the desired niche, and provide a suitable environment for stem cell EXPANSION and differentiation.

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APA: Copy

VAZIFEH SHIRAN, N., SOLEYMANI, MASOUD, MORTAZAVI, YOUSEF, KAVIANI, S., ABOU ALGHASEMI, H., & NIKOU GOFTAR, M.. (2010). 3D CULTURE OF UMBLICAL CORD BLOOD-DRIVED CD₃₄⁺ CELLS ON A DBM SCAFFOLD COATED BY CORD BLOOD–DERIVED UNRESTRICTED SOMATIC STEM CELLS (USSC). PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), 13(3), 63-81. SID. https://sid.ir/paper/81216/en

Vancouver: Copy

VAZIFEH SHIRAN N., SOLEYMANI MASOUD, MORTAZAVI YOUSEF, KAVIANI S., ABOU ALGHASEMI H., NIKOU GOFTAR M.. 3D CULTURE OF UMBLICAL CORD BLOOD-DRIVED CD₃₄⁺ CELLS ON A DBM SCAFFOLD COATED BY CORD BLOOD–DERIVED UNRESTRICTED SOMATIC STEM CELLS (USSC). PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES)[Internet]. 2010;13(3):63-81. Available from: https://sid.ir/paper/81216/en

IEEE: Copy

N. VAZIFEH SHIRAN, MASOUD SOLEYMANI, YOUSEF MORTAZAVI, S. KAVIANI, H. ABOU ALGHASEMI, and M. NIKOU GOFTAR, “3D CULTURE OF UMBLICAL CORD BLOOD-DRIVED CD₃₄⁺ CELLS ON A DBM SCAFFOLD COATED BY CORD BLOOD–DERIVED UNRESTRICTED SOMATIC STEM CELLS (USSC),” PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), vol. 13, no. 3, pp. 63–81, 2010, [Online]. Available: https://sid.ir/paper/81216/en

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