DIFFERENTIATION OF MOUSE EMBRYONIC STEM CELLS INTO HEMATOPOIETIC CELLS

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SOLEYMANI M.; ATASHI A.; MASOUMI M.; BAHARVAND H.

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Journal: Anatomical Sciences Journal Year:2005 | Volume:3 | Issue:1 Page(s): 11-18

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Title

DIFFERENTIATION OF MOUSE EMBRYONIC STEM CELLS INTO HEMATOPOIETIC CELLS

Author(s)

SOLEYMANI M. | ATASHI A. | MASOUMI M. | BAHARVAND H. | Issue Writer Certificate

Keywords

MOUSE EMB BRYONIC STEM CELLQ4

HEMATOPOIETIC CELLSQ4

DIFFERENTIATIONQ3

Abstract

Purpose: DIFFERENTIATION of Mouse embryonicstem cells into HEMATOPOIETIC CELLS.Materials and Methods: In this study, we used EB formation system for Hematopoietic DIFFERENTIATION of mouse embryonic stem cell (Royan B1) in suspension culture. EBs cultured in medium with Hematopoietic inducer cytokines (SCF, TPO, GMCSF, IL3, Flt3 and EPO) .presence of hematopoietic differentiated cell assessed by RT-PCR and colony forming assay.Results: RT-PCR results showed the expression of two chain of mouse hemoglobin in differentiated cell. Also, colony forming assay confirmed results of RT-PCR. Colony of HEMATOPOIETIC CELLS and benzidine positive cells that are representative of erythroid colonies were seen in semisolid media.Conclusion: The results of this study showed that cultivation of EBs in suspension medium with hematopoietic inducer cytokines is effective method for DIFFERENTIATION of embryonic stem cells into HEMATOPOIETIC CELLS.

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APA: Copy

SOLEYMANI, M., ATASHI, A., MASOUMI, M., & BAHARVAND, H.. (2005). DIFFERENTIATION OF MOUSE EMBRYONIC STEM CELLS INTO HEMATOPOIETIC CELLS. JOURNAL OF IRANIAN ANATOMICAL SCIENCES, 3(1), 11-18. SID. https://sid.ir/paper/81489/en

Vancouver: Copy

SOLEYMANI M., ATASHI A., MASOUMI M., BAHARVAND H.. DIFFERENTIATION OF MOUSE EMBRYONIC STEM CELLS INTO HEMATOPOIETIC CELLS. JOURNAL OF IRANIAN ANATOMICAL SCIENCES[Internet]. 2005;3(1):11-18. Available from: https://sid.ir/paper/81489/en

IEEE: Copy

M. SOLEYMANI, A. ATASHI, M. MASOUMI, and H. BAHARVAND, “DIFFERENTIATION OF MOUSE EMBRYONIC STEM CELLS INTO HEMATOPOIETIC CELLS,” JOURNAL OF IRANIAN ANATOMICAL SCIENCES, vol. 3, no. 1, pp. 11–18, 2005, [Online]. Available: https://sid.ir/paper/81489/en

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