EFFECTS OF DIFFERENT CONCENTRATIONS OF MORPHINE ON STAUROSPORINE-INDUCED NEURITE OUTGROWTH IN PC12 CELLS

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ZHALEH HOSSEIN; AZADBAKHT M.; BIDMESHKIPOUR A.; HARAMSHAHI S.M.A.; KEHTARI M.

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Journal: Anatomical Sciences Journal Year:2012 | Volume:9 | Issue:37 Page(s): 318-328

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Title

EFFECTS OF DIFFERENT CONCENTRATIONS OF MORPHINE ON STAUROSPORINE-INDUCED NEURITE OUTGROWTH IN PC12 CELLS

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Keywords

MORPHINEQ3

STAUROSPORINEQ2

NEURITE OUTGROWTHQ2

PC12Q3

Abstract

Purpose: The present study was conducted to evaluate the effect of different concentrations of MORPHINE on STAUROSPORINE-induced NEURITE OUTGROWTH in PC12 cells.Materials and Methods: PC12 cells were cultured in RPMI1640 culture medium supplemented with 0.2% BSA. Cells were divided into three groups; Ι, ΙΙ and ΙΙΙ, culture in the presence of 50, 100 and 214 nM STAUROSPORINE respectively. In each group cells were treated with different concentrations (10-12, 10-10, 10-8, 10-6, 10-4 and 0.0 M) of MORPHINE; treatments 1, 2, 3, 4, 5 and 6 respectively. The percentage of cytotoxicity was performed by lactate dehydrogenase assay. The percentage of death cell was assessed by Hoechst/PI differential staining. Total neurite length was measured.Results: In all groups, the percentage of cytotoxicity as well as the percentage of cell death in treatments 4 and 5 was higher and in treatments 1 and 2 was lower than that of treatment 6 (p<0.05). In group ΙΙΙ, total neurite length in treatments 1 and 2 was higher than that of treatment 6 and in group I, total neurite length in treatments 4 and 5 was lower than treatment 6 (p<0.05). Opioid receptor antagonist (naltrexone) was caused reversed the effects of MORPHINE (p<0.05).Conclusion: Our results suggest that MORPHINE at low concentrations should improve STAUROSPORINE-induced NEURITE OUTGROWTH in PC12 cells.

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APA: Copy

ZHALEH, HOSSEIN, AZADBAKHT, M., BIDMESHKIPOUR, A., HARAMSHAHI, S.M.A., & KEHTARI, M.. (2012). EFFECTS OF DIFFERENT CONCENTRATIONS OF MORPHINE ON STAUROSPORINE-INDUCED NEURITE OUTGROWTH IN PC12 CELLS. JOURNAL OF IRANIAN ANATOMICAL SCIENCES, 9(37), 318-328. SID. https://sid.ir/paper/81516/en

Vancouver: Copy

ZHALEH HOSSEIN, AZADBAKHT M., BIDMESHKIPOUR A., HARAMSHAHI S.M.A., KEHTARI M.. EFFECTS OF DIFFERENT CONCENTRATIONS OF MORPHINE ON STAUROSPORINE-INDUCED NEURITE OUTGROWTH IN PC12 CELLS. JOURNAL OF IRANIAN ANATOMICAL SCIENCES[Internet]. 2012;9(37):318-328. Available from: https://sid.ir/paper/81516/en

IEEE: Copy

HOSSEIN ZHALEH, M. AZADBAKHT, A. BIDMESHKIPOUR, S.M.A. HARAMSHAHI, and M. KEHTARI, “EFFECTS OF DIFFERENT CONCENTRATIONS OF MORPHINE ON STAUROSPORINE-INDUCED NEURITE OUTGROWTH IN PC12 CELLS,” JOURNAL OF IRANIAN ANATOMICAL SCIENCES, vol. 9, no. 37, pp. 318–328, 2012, [Online]. Available: https://sid.ir/paper/81516/en

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