THE EFFECTS OF PLLA NANOFIBER SCAFFOLD ON PROLIFERATION OF FROZEN-THAWED NEONATE MOUSE SPERMATOGONIAL STEM CELLS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

SLAHI N.; HADJIGHASSEM M.R.; JOGHATAEI M.T.; BAKHTIYARI M.; AYYOUBIYAN M.; ASADI M.H.; KORUJI M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Anatomical Sciences Journal Year:2012 | Volume:9 | Issue:37 Page(s): 280-294

Download Full-Text

Persian Verion

View:

864

Download:

Cites:

Information Journal Paper

Title

THE EFFECTS OF PLLA NANOFIBER SCAFFOLD ON PROLIFERATION OF FROZEN-THAWED NEONATE MOUSE SPERMATOGONIAL STEM CELLS

Author(s)

Keywords

SPERMATOGONIL STEM CELLSQ2

PLLA NANO FIBERSQ2

TESTISQ3

Abstract

Purpose: To investigate of the effects of a poly L-lactic acid (PLLA) nanofiber scaffold on proliferation of frozen-thawed neonate mouse spermatogonial stem cells.Materials and Methods: Spermatogonial cells were isolated from neonatal 3-6-day-old NMRI mice testes by two steps enzymatic digestion and differential plating. The isolated spermatogonial cells were divided into four culture groups: 1) fresh spermatogonial cells, 2) fresh spermatogonial cells seeded onto PLLA 3) frozen-thawed spermatogonial cells, 4) frozen-thawed spermatogonial cells seeded onto PLLA. Cells in all groups were cultured in DMEM supplemented with 5% FCS and 10 ng/ml GDNF for 3 weeks. Diameter and number of clusters which were determined during the culture and semi-quantitative RT-PCR were carried out at the end of 3rd week for all culture groups. Presence of spermatogonia at the culture was determined by reverse transcription polymerase chain reaction (RT-PCR) for several important spermatogonial markers (PLZF, Oct4, GFRα-1, VASA, ITGA6 and ITGB1). The significancy of the data was analyzed using Repeated Measures and ANOVA tests.Results: The findings indicated that the viability rate of the fresh cell (control 1 and exprimental 1) and the frozen cells after thawing (control 2 and exprimental 2) were 89.25±2.2 and 63±3.56, respectively and the differences were significant (p<0.001). In vitro culturing of spermatogonial cells on PLLA significantly increased the formation of cell clusters in comparison with those of the control groups (p£0.001). Although the differences of the diameters of clusters in the fresh cell groups were not significant, culturing of frozen-thawed cells on PLLA significantly decreased their diameters (p£0.01). There was a significant down-regulation of spermatogonial genes in the frozenthawed groups after three weeks of culture.Conclusions: The spermatogonial cells seeding on PLLA can increase in vitro cluster formation of neonate fresh and frozen-thawed spermatogonial cells.

Cites

No record.

References

No record.

Cite

APA: Copy

SLAHI, N., HADJIGHASSEM, M.R., JOGHATAEI, M.T., BAKHTIYARI, M., AYYOUBIYAN, M., ASADI, M.H., & KORUJI, M.. (2012). THE EFFECTS OF PLLA NANOFIBER SCAFFOLD ON PROLIFERATION OF FROZEN-THAWED NEONATE MOUSE SPERMATOGONIAL STEM CELLS. JOURNAL OF IRANIAN ANATOMICAL SCIENCES, 9(37), 280-294. SID. https://sid.ir/paper/81520/en

Vancouver: Copy

SLAHI N., HADJIGHASSEM M.R., JOGHATAEI M.T., BAKHTIYARI M., AYYOUBIYAN M., ASADI M.H., KORUJI M.. THE EFFECTS OF PLLA NANOFIBER SCAFFOLD ON PROLIFERATION OF FROZEN-THAWED NEONATE MOUSE SPERMATOGONIAL STEM CELLS. JOURNAL OF IRANIAN ANATOMICAL SCIENCES[Internet]. 2012;9(37):280-294. Available from: https://sid.ir/paper/81520/en

IEEE: Copy

N. SLAHI, M.R. HADJIGHASSEM, M.T. JOGHATAEI, M. BAKHTIYARI, M. AYYOUBIYAN, M.H. ASADI, and M. KORUJI, “THE EFFECTS OF PLLA NANOFIBER SCAFFOLD ON PROLIFERATION OF FROZEN-THAWED NEONATE MOUSE SPERMATOGONIAL STEM CELLS,” JOURNAL OF IRANIAN ANATOMICAL SCIENCES, vol. 9, no. 37, pp. 280–294, 2012, [Online]. Available: https://sid.ir/paper/81520/en

Related Journal Papers