EFFECT OF NEUROTROPHIN-3 ON DIFFERENTIATION OF RAT HAIR FOLLICLE STEM CELLS INTO NEURAL LIKE CELLS

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MOGHANI GHOROGHI F.; NOBAKHT M.; ESMAEILZADE B.; BAKHTIYARI M.; HEJAZIAN L.B.

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Journal: Anatomical Sciences Journal Year:2012 | Volume:9 | Issue:37 Page(s): 270-278

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Title

EFFECT OF NEUROTROPHIN-3 ON DIFFERENTIATION OF RAT HAIR FOLLICLE STEM CELLS INTO NEURAL LIKE CELLS

Author(s)

Keywords

BULGE OF HAIR FOLLICLEQ2

CD34Q2

NESTINQ3

NT-3Q2

ΒⅢ-TUBULINQ2

Abstract

Purpose: The aim of this study was to evaluate the effect of NT-3 on the decrease of the differentiation time of bulge stem cells of rat hair follicle from neuron like cells.Materials and Methods: The bulge region of the rat whisker was isolated from and cultured in DMEM/F12 supplemented with epidermal growth factor (EGF) in 3 groups for 7, 8 and 9 days .Then 10 ng/ml NT-3 was added to each group for 3 days. Finally, cell differentiation was assessed by immunocytochemistry using ΒⅢ-TUBULIN antibody and the result was compared with control groups.Results: According to our results hair follicle bulge stem cells expressed CD34 and NESTIN in 7-9 and 10-12 first days after cultivation respectively. By using NT-3 duration differentiation process the cells showed positive reaction to ΒⅢ-TUBULIN antibody.Conclusion: The results indicate that NT-3 can affect on differentiation speed even in less than 10 days.

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APA: Copy

MOGHANI GHOROGHI, F., NOBAKHT, M., ESMAEILZADE, B., BAKHTIYARI, M., & HEJAZIAN, L.B.. (2012). EFFECT OF NEUROTROPHIN-3 ON DIFFERENTIATION OF RAT HAIR FOLLICLE STEM CELLS INTO NEURAL LIKE CELLS. JOURNAL OF IRANIAN ANATOMICAL SCIENCES, 9(37), 270-278. SID. https://sid.ir/paper/81522/en

Vancouver: Copy

MOGHANI GHOROGHI F., NOBAKHT M., ESMAEILZADE B., BAKHTIYARI M., HEJAZIAN L.B.. EFFECT OF NEUROTROPHIN-3 ON DIFFERENTIATION OF RAT HAIR FOLLICLE STEM CELLS INTO NEURAL LIKE CELLS. JOURNAL OF IRANIAN ANATOMICAL SCIENCES[Internet]. 2012;9(37):270-278. Available from: https://sid.ir/paper/81522/en

IEEE: Copy

F. MOGHANI GHOROGHI, M. NOBAKHT, B. ESMAEILZADE, M. BAKHTIYARI, and L.B. HEJAZIAN, “EFFECT OF NEUROTROPHIN-3 ON DIFFERENTIATION OF RAT HAIR FOLLICLE STEM CELLS INTO NEURAL LIKE CELLS,” JOURNAL OF IRANIAN ANATOMICAL SCIENCES, vol. 9, no. 37, pp. 270–278, 2012, [Online]. Available: https://sid.ir/paper/81522/en

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