EFFECT OF FIBROBLASTIC GROWTH FACTOR ON RESUMPTION OF MEIOSIS, IN VITRO MATURATION AND EMBRYO DEVELOPMENT OF IMMATURE MOUSE OOCYTES

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AZARNIA MAHNAZ; GHASEMIAN F.; BAHADORI M.H.; MOHAMMAD GHASEMI F.; AHMADI JALALI MOGHADAM M.

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Journal: Anatomical Sciences Journal Year:2009 | Volume:7 | Issue:27 Page(s): 1-10

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Title

EFFECT OF FIBROBLASTIC GROWTH FACTOR ON RESUMPTION OF MEIOSIS, IN VITRO MATURATION AND EMBRYO DEVELOPMENT OF IMMATURE MOUSE OOCYTES

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FIBROBLAST GROWTH FACTORQ3

IVMQ3

IVFQ3

EMBRYO DEVELOPMENTQ2

Abstract

Purpose: The purpose of this study was to evaluate the effect of fibroblastic growth factor on resumption of meiosis, in vitro maturation of immature mouse oocytes and resulting EMBRYO DEVELOPMENT with and without basic fibroblastic growth factor-4 (bFGF-4).Materials and Methods: cumulus – oocyte complex (COCs) and germinal vesicle (GV) were obtained from female NMRI mice 46-48 hours after administration of an intra-peritoneal injection of 5 IU PMSG. COCs were cultured in TCM199 supplemented with different dosages of bFGF-4. After 24 hours, metaphase II (MII) oocytes were co-incubated with sperms for 4-6 hours in T6 medium. For all groups, the rate of cleaved embryos was assessed in the T6 medium until blastocyst stage.Results: In all compared groups, the percentage of matured MII oocytes in the10 ng/ml (%94.4) and 20 ng/ml(%92.5) of bFGF-4 treatment groups, was significantly higher (P<0.05) than those of the control group but the percentage of embryos that developed to blastocyst in 20 ng/ml bFGF-4 treatment group was significantly higher than those of the control group (P<0.05).Conclusion: Exogenous bFGF-4 improved the oocyte maturation and EMBRYO DEVELOPMENT.

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APA: Copy

AZARNIA, MAHNAZ, GHASEMIAN, F., BAHADORI, M.H., MOHAMMAD GHASEMI, F., & AHMADI JALALI MOGHADAM, M.. (2009). EFFECT OF FIBROBLASTIC GROWTH FACTOR ON RESUMPTION OF MEIOSIS, IN VITRO MATURATION AND EMBRYO DEVELOPMENT OF IMMATURE MOUSE OOCYTES. JOURNAL OF IRANIAN ANATOMICAL SCIENCES, 7(27), 1-10. SID. https://sid.ir/paper/81548/en

Vancouver: Copy

AZARNIA MAHNAZ, GHASEMIAN F., BAHADORI M.H., MOHAMMAD GHASEMI F., AHMADI JALALI MOGHADAM M.. EFFECT OF FIBROBLASTIC GROWTH FACTOR ON RESUMPTION OF MEIOSIS, IN VITRO MATURATION AND EMBRYO DEVELOPMENT OF IMMATURE MOUSE OOCYTES. JOURNAL OF IRANIAN ANATOMICAL SCIENCES[Internet]. 2009;7(27):1-10. Available from: https://sid.ir/paper/81548/en

IEEE: Copy

MAHNAZ AZARNIA, F. GHASEMIAN, M.H. BAHADORI, F. MOHAMMAD GHASEMI, and M. AHMADI JALALI MOGHADAM, “EFFECT OF FIBROBLASTIC GROWTH FACTOR ON RESUMPTION OF MEIOSIS, IN VITRO MATURATION AND EMBRYO DEVELOPMENT OF IMMATURE MOUSE OOCYTES,” JOURNAL OF IRANIAN ANATOMICAL SCIENCES, vol. 7, no. 27, pp. 1–10, 2009, [Online]. Available: https://sid.ir/paper/81548/en

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