DIFFERENTIATION OF SOMATIC UNRESTRICTED CORD BLOOD STEM CELLS INTO CHONDROCYTE CELLS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

NEZHAD DEHBASHI F.; SOLEYMANI MASOUD; KAVIANI SAEID; ATASHI AMIR; HEYDARI SAEID

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Anatomical Sciences Journal Year:2008 | Volume:6 | Issue:23 Page(s): 353-362

Download Full-Text

Persian Verion

View:

1,345

Download:

Cites:

Information Journal Paper

Title

DIFFERENTIATION OF SOMATIC UNRESTRICTED CORD BLOOD STEM CELLS INTO CHONDROCYTE CELLS

Author(s)

Keywords

CHONDROGENESISQ2

UNRESTRICTED SOMATIC STEM CELLQ3

TGF-β1Q3

TISSUE ENGINEERINGQ3

Abstract

Purpose: To isolate and purify unrestricted somatic stem cells from human umbilical cord blood and evaluation of their differentiation into chondrocyte in vitro.Materials and Methods: In this study cells from human umbilical cord blood were isolated and plated in flask. Colonies were performed after one week. To determine the kind of cells, 100000 cells were analysed with flowcytometry. Twenty thousands (200000) of cells were plated in 6 wells that coated with poly-L-lysine II and incubated in chondrogenic medium to analyze the differentiation of these cells into cartilage. After 24 hs the first pletted cells were formed that continued to be existing to 21 days. The culture of cells were exchanged into chondrogenic culture every two days. At the end of differentiation period and 3 weeks the cells were analyzed by Alcian blue, immunohistichemistry and RT-PCR In addition these cells were passaged 50 times and their karyotyping analyzed.Results: In early days of primary cultures , the number of spindle cells were increased and almost purified in second passage. The differentiation by RT-PCR analysis showed high production of collagen II , aggrican, BMP-6 and collagen that all are the specific genes of chondrocye cells. and.histochemistry assay showed that the methachromatic matrix was accumulated between the cells and expression of collagenII was confirmed. Karyotyping analysis showed high passages for these cells that was expected.Conclusion: Cultured USSC Differentiated into a chondroblast cell linage potential source for cell transplantation for roumatoied arthirits as soon as cord blood is better source for mesenchymal Stem cells against bone marrow.

Cites

No record.

References

No record.

Cite

APA: Copy

NEZHAD DEHBASHI, F., SOLEYMANI, MASOUD, KAVIANI, SAEID, ATASHI, AMIR, & HEYDARI, SAEID. (2008). DIFFERENTIATION OF SOMATIC UNRESTRICTED CORD BLOOD STEM CELLS INTO CHONDROCYTE CELLS. JOURNAL OF IRANIAN ANATOMICAL SCIENCES, 6(23), 353-362. SID. https://sid.ir/paper/81561/en

Vancouver: Copy

NEZHAD DEHBASHI F., SOLEYMANI MASOUD, KAVIANI SAEID, ATASHI AMIR, HEYDARI SAEID. DIFFERENTIATION OF SOMATIC UNRESTRICTED CORD BLOOD STEM CELLS INTO CHONDROCYTE CELLS. JOURNAL OF IRANIAN ANATOMICAL SCIENCES[Internet]. 2008;6(23):353-362. Available from: https://sid.ir/paper/81561/en

IEEE: Copy

F. NEZHAD DEHBASHI, MASOUD SOLEYMANI, SAEID KAVIANI, AMIR ATASHI, and SAEID HEYDARI, “DIFFERENTIATION OF SOMATIC UNRESTRICTED CORD BLOOD STEM CELLS INTO CHONDROCYTE CELLS,” JOURNAL OF IRANIAN ANATOMICAL SCIENCES, vol. 6, no. 23, pp. 353–362, 2008, [Online]. Available: https://sid.ir/paper/81561/en

Related Journal Papers