EFFECT OF Β-MERCAPTOETHANOLON RESUMPTION OF MEIOSIS, IN VITRO MATURATION AND DEVELOPMENT OF IMMATURE MOUSE OOCYTE TREATED WITH DEHP (DI-(2-ETHYLHEXY) PHATHALAE)

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IMANI H.; DALMAN A.; SEPEHRI H.; KAZEMI S.; HASANI F.; SHAH VERDI A.H.; BAHARVAND H.; REZAZADEH M.

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Journal: Anatomical Sciences Journal Year:2005 | Volume:3 | Issue:2 Page(s): 109-116

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Title

EFFECT OF Β-MERCAPTOETHANOLON RESUMPTION OF MEIOSIS, IN VITRO MATURATION AND DEVELOPMENT OF IMMATURE MOUSE OOCYTE TREATED WITH DEHP (DI-(2-ETHYLHEXY) PHATHALAE)

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Keywords

IMMATURE OOCYTEQ3

IN VITRO MATURATIONQ4

MOUSEQ3

Β-MERCAPTOETHANOLQ4

DEHP (DI-(2-ETHYLHEXY) PHATHALAE)Q4

Abstract

Purpose: The aim of this study was evaluated the effect of β -mercaptoethanol on resumption of meiosis, In vivo maturation of immature MOUSE oocytes treated with DEHP and embryo development.Materials and Methods: NMRI MOUSE strain (4-6 weeks) were treated daily with 50,µI DEHP Group 1: 121 IMMATURE OOCYTEs were matured in MEM-α medium contain FCS 5%, hCG 7.5 IU/ml, rFsH 100mIU/ml, Group 2: 120 IMMATURE OOCYTEs were matured in MEMα medium contain FCS 5%, hCG 7.5IU/ml, rFsH 100mIU/ml and 100µm Β-MERCAPTOETHANOL.Control group: 198 immature MOUSE oocytes recovered from nontreated MOUSE and were matured in MEMα medium contain FCS 5%, hCG 7.5IU/ml, rFSH 100mlU/ml. Fertilization and embryo development were done in T6 medium.Results: The resumption of meiosis in experimental groups (1&2) were (71%, 79%) respectively. However the resumption of meiosis in experimental groups were significantly different compared to control group (89%) (p<0.05). The IN VITRO MATURATION in experimental groups (1,2) were (52%, 68%) respectively. However the IN VITRO MATURATION in one group was significantly different compared to control group (75%) (p=0.00) and second group was significantly higher than one experimental group (p<0.01).The embryo number, after 24th insemination in experimental groups (1&2) were (52%, 70%) respectively However the embryo number in first group was significantly lower than the control group (74%) (P<0.05) and second group was significantly higher than the first group (p<0.05).Conclusion: The results of this study showed that the Β-MERCAPTOETHANOL (100µM) inhibited the negative effect of DEHP on IN VITRO MATURATION and embryo development. The Β-MERCAPTOETHANOL has improved the resumption of meiosis, In vito maturation and embryo development.

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APA: Copy

IMANI, H., DALMAN, A., SEPEHRI, H., KAZEMI, S., HASANI, F., SHAH VERDI, A.H., BAHARVAND, H., & REZAZADEH, M.. (2005). EFFECT OF Β-MERCAPTOETHANOLON RESUMPTION OF MEIOSIS, IN VITRO MATURATION AND DEVELOPMENT OF IMMATURE MOUSE OOCYTE TREATED WITH DEHP (DI-(2-ETHYLHEXY) PHATHALAE). JOURNAL OF IRANIAN ANATOMICAL SCIENCES, 3(2), 109-116. SID. https://sid.ir/paper/81572/en

Vancouver: Copy

IMANI H., DALMAN A., SEPEHRI H., KAZEMI S., HASANI F., SHAH VERDI A.H., BAHARVAND H., REZAZADEH M.. EFFECT OF Β-MERCAPTOETHANOLON RESUMPTION OF MEIOSIS, IN VITRO MATURATION AND DEVELOPMENT OF IMMATURE MOUSE OOCYTE TREATED WITH DEHP (DI-(2-ETHYLHEXY) PHATHALAE). JOURNAL OF IRANIAN ANATOMICAL SCIENCES[Internet]. 2005;3(2):109-116. Available from: https://sid.ir/paper/81572/en

IEEE: Copy

H. IMANI, A. DALMAN, H. SEPEHRI, S. KAZEMI, F. HASANI, A.H. SHAH VERDI, H. BAHARVAND, and M. REZAZADEH, “EFFECT OF Β-MERCAPTOETHANOLON RESUMPTION OF MEIOSIS, IN VITRO MATURATION AND DEVELOPMENT OF IMMATURE MOUSE OOCYTE TREATED WITH DEHP (DI-(2-ETHYLHEXY) PHATHALAE),” JOURNAL OF IRANIAN ANATOMICAL SCIENCES, vol. 3, no. 2, pp. 109–116, 2005, [Online]. Available: https://sid.ir/paper/81572/en

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