PREPARATION OF HUMAN 3D SKIN MATRIX AND INVESTIGATION OF THE INTERACTION BETWEEN RAT ADHERENT BONE MARROW CELLS AND PREPARED MATRIX

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IZI LEILA; LARI ROYA; MAHDAVI SHAHRI NASSER; ROJHAN NEZHAD MEHRNEGAR; BIRJANDI NEJAD ALI

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Journal: Journal of Sabzevar University of Medical Sciences Year:2016 | Volume:23 | Issue:2 Page(s): 241-252

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Title

PREPARATION OF HUMAN 3D SKIN MATRIX AND INVESTIGATION OF THE INTERACTION BETWEEN RAT ADHERENT BONE MARROW CELLS AND PREPARED MATRIX

Author(s)

Keywords

EXTRACELLULAR MATRIXQ3

DECELLULARIZATIONQ2

SCAFFOLDQ1

HUMAN SKINQ1

RAT ADHERENT BONE MARROW CELLSQ1

Abstract

Background: Decellularised tissues matrix is considered as an ultimate SCAFFOLDing system for two reasons: firstly it has mechanical and structural similarity with natural tissue, secondly extra cellular matrix proteins remain intact after DECELLULARIZATION, which are important for cell differentiation. The aim of this study is investigation behavior RAT ADHERENT BONE MARROW CELLS cultured in decellularised matrix of HUMAN SKIN.Materials and Methods: In this study, HUMAN SKIN tissues were decellularised by three methods: physical (slow freezing and snap freeze–thaw), enzymatic (trypsin 0.25%, 18 hours) and chemical (sodium dodecyl sulfate- SDS 1%, 12 hours). After washing and sterilization procedures, SCAFFOLDs cultivated with 4´105 RAT ADHERENT BONE MARROW CELLS. Finally, histological studies were performed after 1, 2, 3 and 4 weeks of culture and investigated with different staining.Results: Histological study of decellularised SCAFFOLDs revealed that nuclear and cellular components of the tissues were completely removed and collagen fibers remained intact. At 1, 2, 3 and 4 weeks of culture, in vitro analysis showed adhesion and also proliferation of RAT ADHERENT BONE MARROW CELLS. Also, we did not observe any penetration and migration of the cells into SCAFFOLDs. Statistical analysis indicated significant difference in percentage of cell density in specimens cultured after 2 weeks versus the other specimens (P<0.001).Conclusion: the overall results showed that remnant ECM (EXTRACELLULAR MATRIX) of HUMAN SKIN can be a suitable SCAFFOLD for studying RAT ADHERENT BONE MARROW CELLS behavior.

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References

THREE-DIMENSIONAL SCAFFOLD FROM DECELLULARIZED HUMAN GINGIVA FOR CELL CULTURES: GLYCOCONJUGATES AND CELL BEHAVIOR

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APA: Copy

IZI, LEILA, LARI, ROYA, MAHDAVI SHAHRI, NASSER, ROJHAN NEZHAD, MEHRNEGAR, & BIRJANDI NEJAD, ALI. (2016). PREPARATION OF HUMAN 3D SKIN MATRIX AND INVESTIGATION OF THE INTERACTION BETWEEN RAT ADHERENT BONE MARROW CELLS AND PREPARED MATRIX. JOURNAL OF SABZEVAR UNIVERSITY OF MEDICAL SCIENCES, 23(2), 241-252. SID. https://sid.ir/paper/81956/en

Vancouver: Copy

IZI LEILA, LARI ROYA, MAHDAVI SHAHRI NASSER, ROJHAN NEZHAD MEHRNEGAR, BIRJANDI NEJAD ALI. PREPARATION OF HUMAN 3D SKIN MATRIX AND INVESTIGATION OF THE INTERACTION BETWEEN RAT ADHERENT BONE MARROW CELLS AND PREPARED MATRIX. JOURNAL OF SABZEVAR UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2016;23(2):241-252. Available from: https://sid.ir/paper/81956/en

IEEE: Copy

LEILA IZI, ROYA LARI, NASSER MAHDAVI SHAHRI, MEHRNEGAR ROJHAN NEZHAD, and ALI BIRJANDI NEJAD, “PREPARATION OF HUMAN 3D SKIN MATRIX AND INVESTIGATION OF THE INTERACTION BETWEEN RAT ADHERENT BONE MARROW CELLS AND PREPARED MATRIX,” JOURNAL OF SABZEVAR UNIVERSITY OF MEDICAL SCIENCES, vol. 23, no. 2, pp. 241–252, 2016, [Online]. Available: https://sid.ir/paper/81956/en

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