ASSESSMENT OF ANTIBACTERIAL EFFECT OF NANO POLYAMIDOAMINE-G5 (NPAMAM-G5) DENDRIMER ON ESCHERICHIA COLI, PROTEUS MIRABILIS, SALMONELLA TYPHI, BACILLUS SUBTILIS AND STAPHYLOCOCCUS AUREUS ISOLATED FROM THE HOSPITAL ENVIRONMENT

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NAZARI SHAHRAM; BAGHERI SOSAN; MOHAMMADI LILA; MAJIDI GHARIB; KAMRAN AZIZ; ASGHARI ESRAFIL; Azghani Payman; DARKHOSH ZOHREH; Nazari Zohreh; ALIZADEH MATBOO SOUDABEH

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Journal: Journal of Sabzevar University of Medical Sciences Year:2018 | Volume:24 | Issue:6 Page(s): 112-120

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Title

ASSESSMENT OF ANTIBACTERIAL EFFECT OF NANO POLYAMIDOAMINE-G5 (NPAMAM-G5) DENDRIMER ON ESCHERICHIA COLI, PROTEUS MIRABILIS, SALMONELLA TYPHI, BACILLUS SUBTILIS AND STAPHYLOCOCCUS AUREUS ISOLATED FROM THE HOSPITAL ENVIRONMENT

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ANTIBACTERIAL EFFECTQ2

GRAM NEGATIVE AND GRAM POSITIVE BACTERIAQ1

HOSPITAL ENVIRONMENTQ1

NANO POLYAMIDOAMINE-G5Q1

Abstract

Background & Objectives: This study aimed to examine the ANTIBACTERIAL EFFECT of NANO POLYAMIDOAMINE-G5 (NPAMAM-G5) dendrimer on Escherichia Coli, Proteus Mirabilis, Salmonella Typhi, Bacillus Subtilis and Staphylococcus Aureu s isolated from the HOSPITAL ENVIRONMENT.Materials & Methods: In this study, the ANTIBACTERIAL EFFECTs of NPAMAM-G5 dendrimer were studied by disc diffusion and micro-dilution method. Different concentrations of Polyamidoamine-G5 inoculated onto blank disks and were placed in Mueller-Hinton agar media. Zone of inhibition was investigated by bacterial inoculation according to the McFarland standard 0.5. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of NPAMAM-G5 dendrimer were determined by micro-dilution method.Results: Zone of inhibition in Polyamidoamine-G5 dendrimers concentration of 25 mg/mL for Escherichia Coli, Proteus Mirabilis, Salmonella Typhi, Bacillus Subtilis and Staphylococcus Aureus were 22, 28, 21, 16 and 20 mm, respectively. Regarding the zone of inhibition in gram negative bacteria with gram positive ones was p=0.16 and was not significant different. The minimum inhibitory concentration for both Proteus Mirabilis and Salmonella Typhi was 2.5 mg/mL and for Escherichia Coli, Bacillus Subtilis and Staphylococcus Aureus was 25 mg/mL. The Minimum Bactericidal Concentration (MBC) for Salmonella Typhi was 50 mg/mL and for all Proteus Mirabilis, Escherichia Coli, Bacillus Subtilis and Staphylococcus Aureus was 100 mg/mL.Conclusion: According to the results, NPAMAM-G5 dendrimer can effectively eliminate Escherichia Coli, Proteus Mirabilis, Salmonella Typhi, Bacillus Subtilis and Staphylococcus Aureus.

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APA: Copy

NAZARI, SHAHRAM, BAGHERI, SOSAN, MOHAMMADI, LILA, MAJIDI, GHARIB, KAMRAN, AZIZ, ASGHARI, ESRAFIL, Azghani, Payman, DARKHOSH, ZOHREH, Nazari, Zohreh, & ALIZADEH MATBOO, SOUDABEH. (2018). ASSESSMENT OF ANTIBACTERIAL EFFECT OF NANO POLYAMIDOAMINE-G5 (NPAMAM-G5) DENDRIMER ON ESCHERICHIA COLI, PROTEUS MIRABILIS, SALMONELLA TYPHI, BACILLUS SUBTILIS AND STAPHYLOCOCCUS AUREUS ISOLATED FROM THE HOSPITAL ENVIRONMENT. JOURNAL OF SABZEVAR UNIVERSITY OF MEDICAL SCIENCES, 24(6 ), 112-120. SID. https://sid.ir/paper/82287/en

Vancouver: Copy

NAZARI SHAHRAM, BAGHERI SOSAN, MOHAMMADI LILA, MAJIDI GHARIB, KAMRAN AZIZ, ASGHARI ESRAFIL, Azghani Payman, DARKHOSH ZOHREH, Nazari Zohreh, ALIZADEH MATBOO SOUDABEH. ASSESSMENT OF ANTIBACTERIAL EFFECT OF NANO POLYAMIDOAMINE-G5 (NPAMAM-G5) DENDRIMER ON ESCHERICHIA COLI, PROTEUS MIRABILIS, SALMONELLA TYPHI, BACILLUS SUBTILIS AND STAPHYLOCOCCUS AUREUS ISOLATED FROM THE HOSPITAL ENVIRONMENT. JOURNAL OF SABZEVAR UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2018;24(6 ):112-120. Available from: https://sid.ir/paper/82287/en

IEEE: Copy

SHAHRAM NAZARI, SOSAN BAGHERI, LILA MOHAMMADI, GHARIB MAJIDI, AZIZ KAMRAN, ESRAFIL ASGHARI, Payman Azghani, ZOHREH DARKHOSH, Zohreh Nazari, and SOUDABEH ALIZADEH MATBOO, “ASSESSMENT OF ANTIBACTERIAL EFFECT OF NANO POLYAMIDOAMINE-G5 (NPAMAM-G5) DENDRIMER ON ESCHERICHIA COLI, PROTEUS MIRABILIS, SALMONELLA TYPHI, BACILLUS SUBTILIS AND STAPHYLOCOCCUS AUREUS ISOLATED FROM THE HOSPITAL ENVIRONMENT,” JOURNAL OF SABZEVAR UNIVERSITY OF MEDICAL SCIENCES, vol. 24, no. 6 , pp. 112–120, 2018, [Online]. Available: https://sid.ir/paper/82287/en

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