APPLICATION OF THE STEREOLOGY TECHNIQUE IN EVALUATING THE ADDICTIVE EFFECTS OF MORPHINE ON THE CEREBELLA CORTEX VOLUME IN MALE RAT

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MOHAMMAD GHASEMI F.; DEZFOLYAN A.; MAHMOUDZADEH SAGHEB H.R.; SHOHANI B.

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Journal: Journal of Sabzevar University of Medical Sciences Year:2000 | Volume:7 | Issue:1 (15) Page(s): 3-13

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Title

APPLICATION OF THE STEREOLOGY TECHNIQUE IN EVALUATING THE ADDICTIVE EFFECTS OF MORPHINE ON THE CEREBELLA CORTEX VOLUME IN MALE RAT

Author(s)

MOHAMMAD GHASEMI F. | DEZFOLYAN A. | MAHMOUDZADEH SAGHEB H.R. | SHOHANI B. | Issue Writer Certificate

Keywords

MORPHINE-DEPENDENCEQ3

THE CEREBELLUMQ2

RATQ2

STEREOLOGY CORTEX VOLUMEQ3

Abstract

The addictive effect of morphine on the cortex volume of THE CEREBELLUM is evaluated in this study using unbiased, robust and efficient quantitative. stereological methods. 30 male RATs of N-Mary race, aging 3 months and weighing 109± 3.5 gr, were randomly selected and assigned into three groups, the first group receiving no treatment. The second group received normal-Saline, and the third group received an increasing dose of morphine-saline, 5-25mg for 5 days. Doses of treatment were the same for the second and third groups. The animals were deeply anaesthetized with an injection of Ketamin 12 hours after the last dose of the treatment and after seeing the withdrawal symptoms, via the left ventricle of the heart, they were perused with a 0.9% saline and fixation was done. Then, the Cortex volume of THE CEREBELLUM was determined using stereology and cavalier principles. For this purpose, uniform random sampling, parallel slices and counting lattice points were adopted. The ANDVA was used to compare the statistical differences of the study groups. There were no significant differences noticed in the estimated cortex volumes of the animals of the two groups. Therefore, the increasing dose of morphine injection for five days had no addictive effect on the cortex volume of THE CEREBELLUM in the subjects of this study.

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APA: Copy

MOHAMMAD GHASEMI, F., DEZFOLYAN, A., MAHMOUDZADEH SAGHEB, H.R., & SHOHANI, B.. (2000). APPLICATION OF THE STEREOLOGY TECHNIQUE IN EVALUATING THE ADDICTIVE EFFECTS OF MORPHINE ON THE CEREBELLA CORTEX VOLUME IN MALE RAT. JOURNAL OF SABZEVAR UNIVERSITY OF MEDICAL SCIENCES, 7(1 (15)), 3-13. SID. https://sid.ir/paper/82476/en

Vancouver: Copy

MOHAMMAD GHASEMI F., DEZFOLYAN A., MAHMOUDZADEH SAGHEB H.R., SHOHANI B.. APPLICATION OF THE STEREOLOGY TECHNIQUE IN EVALUATING THE ADDICTIVE EFFECTS OF MORPHINE ON THE CEREBELLA CORTEX VOLUME IN MALE RAT. JOURNAL OF SABZEVAR UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2000;7(1 (15)):3-13. Available from: https://sid.ir/paper/82476/en

IEEE: Copy

F. MOHAMMAD GHASEMI, A. DEZFOLYAN, H.R. MAHMOUDZADEH SAGHEB, and B. SHOHANI, “APPLICATION OF THE STEREOLOGY TECHNIQUE IN EVALUATING THE ADDICTIVE EFFECTS OF MORPHINE ON THE CEREBELLA CORTEX VOLUME IN MALE RAT,” JOURNAL OF SABZEVAR UNIVERSITY OF MEDICAL SCIENCES, vol. 7, no. 1 (15), pp. 3–13, 2000, [Online]. Available: https://sid.ir/paper/82476/en

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