CLONING AND RECOMBINANT EXPRESSION MODIFIED PROTECTIVE ANTIGEN FROM BACILLUS ANTHRACIS IN E. COIL

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ETEMAD AUBI SEYED MASIH; HONARI HOSSEIN

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Journal: Journal of Sabzevar University of Medical Sciences Year:2016 | Volume:23 | Issue:1 Page(s): 95-102

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Title

CLONING AND RECOMBINANT EXPRESSION MODIFIED PROTECTIVE ANTIGEN FROM BACILLUS ANTHRACIS IN E. COIL

Author(s)

ETEMAD AUBI SEYED MASIH | HONARI HOSSEIN | Issue Writer Certificate

Keywords

PROTECTIVE ANTIGENQ1

BACILLUS ANTHRACISQ2

RECOMBINANT PROTEINQ2

Abstract

Background: Anthrax is a common illness between human and animal which the agent of that is BACILLUS ANTHRACIS. The modified PROTECTIVE ANTIGEN cab be used in treatment and vaccination. The aim of this study is the recombinant expression of modified PROTECTIVE ANTIGEN in E. coli.Material and Methods: Gene fragments were amplified with PCR from pXOI and fusion gene from SOEing PCR was cloned in a cloning vector and finally sub cloned in pET28a (+) vector and transferred to E. coli BL21 (DE3) competent cells. Recombinant expression of modified PA was induced by IPTG and after protein purification with affinity chromatography, resulted antigen was injected to mice in 4 repeats. Polyclonal antibodies produced in mice serum was accessed.Results: The modified PROTECTIVE ANTIGEN cloned in expression vector pET28a (+) was confirmed by PCR, enzymatic digestion and sequencing. RECOMBINANT PROTEIN was confirmed by SDS-PAGE and western blot. Serum was separated from mice blood and titre of antibody against modified PA was assessed by indirect ELYSA.Conclusion: resulted protein has high inhibitory property for LF and EF virulence factors. By consideration in PA detection by polyclonal antibody against modified PA from BACILLUS ANTHRACIS, it can be used in for treatment and prophylaxis against anthrax in individual or combination forms with other PA domains.

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APA: Copy

ETEMAD AUBI, SEYED MASIH, & HONARI, HOSSEIN. (2016). CLONING AND RECOMBINANT EXPRESSION MODIFIED PROTECTIVE ANTIGEN FROM BACILLUS ANTHRACIS IN E. COIL. JOURNAL OF SABZEVAR UNIVERSITY OF MEDICAL SCIENCES, 23(1), 95-102. SID. https://sid.ir/paper/82651/en

Vancouver: Copy

ETEMAD AUBI SEYED MASIH, HONARI HOSSEIN. CLONING AND RECOMBINANT EXPRESSION MODIFIED PROTECTIVE ANTIGEN FROM BACILLUS ANTHRACIS IN E. COIL. JOURNAL OF SABZEVAR UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2016;23(1):95-102. Available from: https://sid.ir/paper/82651/en

IEEE: Copy

SEYED MASIH ETEMAD AUBI, and HOSSEIN HONARI, “CLONING AND RECOMBINANT EXPRESSION MODIFIED PROTECTIVE ANTIGEN FROM BACILLUS ANTHRACIS IN E. COIL,” JOURNAL OF SABZEVAR UNIVERSITY OF MEDICAL SCIENCES, vol. 23, no. 1, pp. 95–102, 2016, [Online]. Available: https://sid.ir/paper/82651/en

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