DIRECT AND INDIRECT REGENERATION OF ADVENTITIOUS SHOOTS IN ORNAMENTAL TULIPA GESNERIANA L. 'APELDOORN' BY USING IN VITRO CULTURE METHOD

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TAGHI ZADEH MINA; BABALAR M.; ZAMANI Z.A.; NADERI R.; ASKARI MOHAMMAD ALI

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Journal: IRANIAN JOURNAL OF AGRICULTURAL SCIENCES (JOURNAL OF AGRICULTURE) Year:2007 | Volume:37 | Issue:6 Page(s): 1031-1039

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Title

DIRECT AND INDIRECT REGENERATION OF ADVENTITIOUS SHOOTS IN ORNAMENTAL TULIPA GESNERIANA L. 'APELDOORN' BY USING IN VITRO CULTURE METHOD

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Keywords

TULIPQ3

DIRECT AND INDIRECT REGENERATIONQ2

ADVENTITIOUS SHOOTSQ3

NAAQ4

BAPQ2

Abstract

One of the in vitro culture goals in plants is their micro propagation under asceptic conditions. TULIP is one of the top ten cut flowers in the world whose origin is the center of Asia, especially Iran and Turkey. With regard to low speed of traditional propagation of TULIP, in vitro culture with the purpose of increasing propagation speed will have huge economical potential. The goal of this investigation was shoot production through DIRECT AND INDIRECT REGENERATION. It was performed as a factorial, complete randomized design with 3 replications, as well as 13 observations in each plot. Explants (Scales) were obtained from TULIP bulbs, variety TULIPa gesneriana L.'Apeldoorn'. The obtained calluses were subcultured onto MS solid media in order to induce indirect shoot regeneration. Hormone treatments were applied in two separatetrials. In the first trial, NAA of two concentrations (0.05 and 0.5 mg/l) among with BAP at two concentrations (1 and 2 mg/l) were applied. In the second trial NAA at two concentrations (1 and 2 mg/l) in composition with BAP at three concentrations (0, 0.5 and 1 mg/l) were applied. The highest shoot production in the first trial was obtained when applying 0.5 mg/I NAA and 2 mg/1 of BAP. In the second trial 1 mg/l NAA and 2 mg/l BAP caused the highest regeneration in TULIP. In direct regeneration, immature petioles of the same TULIP were cultured onto MS medum with two hormone compositions (1 mg/l NAA with mg/l BAP, and 2 mg/l NAA with 2 mg/l BAP). The highest shoot production was obtained at 2 mg/l NAA with 2 mg/l BAP concentrations.

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APA: Copy

TAGHI ZADEH, MINA, BABALAR, M., ZAMANI, Z.A., NADERI, R., & ASKARI, MOHAMMAD ALI. (2007). DIRECT AND INDIRECT REGENERATION OF ADVENTITIOUS SHOOTS IN ORNAMENTAL TULIPA GESNERIANA L. 'APELDOORN' BY USING IN VITRO CULTURE METHOD. IRANIAN JOURNAL OF AGRICULTURAL SCIENCES (JOURNAL OF AGRICULTURE), 37(6), 1031-1039. SID. https://sid.ir/paper/8392/en

Vancouver: Copy

TAGHI ZADEH MINA, BABALAR M., ZAMANI Z.A., NADERI R., ASKARI MOHAMMAD ALI. DIRECT AND INDIRECT REGENERATION OF ADVENTITIOUS SHOOTS IN ORNAMENTAL TULIPA GESNERIANA L. 'APELDOORN' BY USING IN VITRO CULTURE METHOD. IRANIAN JOURNAL OF AGRICULTURAL SCIENCES (JOURNAL OF AGRICULTURE)[Internet]. 2007;37(6):1031-1039. Available from: https://sid.ir/paper/8392/en

IEEE: Copy

MINA TAGHI ZADEH, M. BABALAR, Z.A. ZAMANI, R. NADERI, and MOHAMMAD ALI ASKARI, “DIRECT AND INDIRECT REGENERATION OF ADVENTITIOUS SHOOTS IN ORNAMENTAL TULIPA GESNERIANA L. 'APELDOORN' BY USING IN VITRO CULTURE METHOD,” IRANIAN JOURNAL OF AGRICULTURAL SCIENCES (JOURNAL OF AGRICULTURE), vol. 37, no. 6, pp. 1031–1039, 2007, [Online]. Available: https://sid.ir/paper/8392/en

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