COMPARISON BETWEEN POLYVINYL PYRROLIDONE/
NA2SO4 AQUEOUS TWO-PHASE SYSTEMS AND CHROMATOGRAPHIC METHODS FOR PURIFICATION OF RECOMBINANT PHENYLALANINE DEHYDROGENASE

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SHAHBAZ MOHAMMADI H.; OMIDINIA E.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Journal: Journal of Sciences, Islamic Republic of Iran Year:2009 | Volume:20 | Issue:4 Page(s): 311-317

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Information Journal Paper

Title

COMPARISON BETWEEN POLYVINYL PYRROLIDONE/ NA2SO4 AQUEOUS TWO-PHASE SYSTEMS AND CHROMATOGRAPHIC METHODS FOR PURIFICATION OF RECOMBINANT PHENYLALANINE DEHYDROGENASE

Author(s)

SHAHBAZ MOHAMMADI H. | OMIDINIA E. | Issue Writer Certificate

Keywords

COMPARISONQ3

SANS-SERIF"> NA2SO4Q3

PURIFICATIONQ3

PHENYLALANINE DEHYDROGENASE (PHEDH)Q3

POLYVINYL PYRROLIDONE (PVP)Q3

Abstract

Phenylalanine dehydrogenase (PheDH; EC 1.4.1.20) is an important enzyme of amino acid dehydrogenases family that increasingly used as a valuable biocatalyst in neonatal screening kits and synthesis of L-phenylalanine. The goal of this literature was to find a suitable PURIFICATION method for recombinant Bacillus badius PheDH by practical COMPARISON between chromatographic and polyvinyl pyrrolidone (PVP)/Na2SO4 aqueous two-phase systems (ATPS) techniques. The partitioning behavior of target enzyme in PVP/Na2SO4 ATPS was examined and compared with the obtained results from a chromatographic protocol. Direct COMPARISON of chromatography and ATPS procedures clearly revealed that the ATPS consisting of 8.0% (w/w) PVP, 17.0% (w/w) Na2SO4 with pH of 8.0, VR=0.25 and temperature of 25oC was the most desirable process for PheDH PURIFICATION. A specific activity of 1231.42 U/mg, a PURIFICATION factor of 36.61, a ield of 95.5% and a recovery of 138.9% were achieved. Altogether, we presented a two-phase methodology as a scalable and economically alternative for the production of PheDH enzyme.

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APA: Copy

SHAHBAZ MOHAMMADI, H., & OMIDINIA, E.. (2009). COMPARISON BETWEEN POLYVINYL PYRROLIDONE/ NA₂SO₄ AQUEOUS TWO-PHASE SYSTEMS AND CHROMATOGRAPHIC METHODS FOR PURIFICATION OF RECOMBINANT PHENYLALANINE DEHYDROGENASE. JOURNAL OF SCIENCES ISLAMIC REPUBLIC OF IRAN, 20(4), 311-317. SID. https://sid.ir/paper/84141/en

Vancouver: Copy

SHAHBAZ MOHAMMADI H., OMIDINIA E.. COMPARISON BETWEEN POLYVINYL PYRROLIDONE/ NA₂SO₄ AQUEOUS TWO-PHASE SYSTEMS AND CHROMATOGRAPHIC METHODS FOR PURIFICATION OF RECOMBINANT PHENYLALANINE DEHYDROGENASE. JOURNAL OF SCIENCES ISLAMIC REPUBLIC OF IRAN[Internet]. 2009;20(4):311-317. Available from: https://sid.ir/paper/84141/en

IEEE: Copy

H. SHAHBAZ MOHAMMADI, and E. OMIDINIA, “COMPARISON BETWEEN POLYVINYL PYRROLIDONE/ NA₂SO₄ AQUEOUS TWO-PHASE SYSTEMS AND CHROMATOGRAPHIC METHODS FOR PURIFICATION OF RECOMBINANT PHENYLALANINE DEHYDROGENASE,” JOURNAL OF SCIENCES ISLAMIC REPUBLIC OF IRAN, vol. 20, no. 4, pp. 311–317, 2009, [Online]. Available: https://sid.ir/paper/84141/en

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