EVIDENCE FOR THE ESSENTIAL ARGININE AND HISTIDINE RESIDUES IN CATALYTIC ACTIVITY OF GLUCOSE 6-PHOSPHATE DEHYDROGENASE FROM STREPTOMYCES AUREOFACIENS

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HAGHIGHI B.; DEHGHANI M.

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Journal: Journal of Sciences, Islamic Republic of Iran Year:2005 | Volume:16 | Issue:1 Page(s): 15-20

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Title

EVIDENCE FOR THE ESSENTIAL ARGININE AND HISTIDINE RESIDUES IN CATALYTIC ACTIVITY OF GLUCOSE 6-PHOSPHATE DEHYDROGENASE FROM STREPTOMYCES AUREOFACIENS

Author(s)

HAGHIGHI B. | DEHGHANI M. | Issue Writer Certificate

Keywords

GLUCOSE 6-PHOSPHATE DEHYDROGENASE; ESSENTIAL AMINO ACIDSQ4

Abstract

Glucose 6-phosphate dehydrogenase (G6PD) was purified from Streptomyces aureofaciens and inactivated with butanedione and diethylpyrocarbonate. Incubation of the enzyme with butanedione resulted in a rapid activity loss (80%) within 5 min, followed by a glow phase using a molar ratio to enzyme concentration of 100. Fluorescence studies showed a conformational change in the butanedione-modified enzyme. NAD+, NADP+ and glucose 6-phosphate protected the enzyme against inactivation. Diethylpyrocarbonate (2 mM) completely inactivated the enzyme after 2 min. Stoichiometry of the inactivation showed 2 moles of histidine "residues per mole of enzyme with complete activity loss. Maximum emission spectrum of the enzyme decreased (23%) upon modification and the presence of NAD+ or NADP+ further decreased the fluorescence by 27% and 10.5%, respective1y. The data suggest that essential arginine and histidine residues may be involved in the catalytic activity of Streptomyces aureofaciens G6PD.

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APA: Copy

HAGHIGHI, B., & DEHGHANI, M.. (2005). EVIDENCE FOR THE ESSENTIAL ARGININE AND HISTIDINE RESIDUES IN CATALYTIC ACTIVITY OF GLUCOSE 6-PHOSPHATE DEHYDROGENASE FROM STREPTOMYCES AUREOFACIENS. JOURNAL OF SCIENCES ISLAMIC REPUBLIC OF IRAN, 16(1), 15-20. SID. https://sid.ir/paper/84180/en

Vancouver: Copy

HAGHIGHI B., DEHGHANI M.. EVIDENCE FOR THE ESSENTIAL ARGININE AND HISTIDINE RESIDUES IN CATALYTIC ACTIVITY OF GLUCOSE 6-PHOSPHATE DEHYDROGENASE FROM STREPTOMYCES AUREOFACIENS. JOURNAL OF SCIENCES ISLAMIC REPUBLIC OF IRAN[Internet]. 2005;16(1):15-20. Available from: https://sid.ir/paper/84180/en

IEEE: Copy

B. HAGHIGHI, and M. DEHGHANI, “EVIDENCE FOR THE ESSENTIAL ARGININE AND HISTIDINE RESIDUES IN CATALYTIC ACTIVITY OF GLUCOSE 6-PHOSPHATE DEHYDROGENASE FROM STREPTOMYCES AUREOFACIENS,” JOURNAL OF SCIENCES ISLAMIC REPUBLIC OF IRAN, vol. 16, no. 1, pp. 15–20, 2005, [Online]. Available: https://sid.ir/paper/84180/en

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