ISOLATION OF ESCHERICHIA COLI O157:H7 FROM GROUND BEEF SAMPLES COLLECTED FROM BEEF MARKETS, USING CONVENTIONAL CULTURE AND POLYMERASE CHAIN REACTION IN MASHHAD, NORTHEASTERN IRAN

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JAMSHIDI ABD ELAH; BASAMI M.R.; RASOULI MARYAM

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Journal: IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR) Year:2008 | Volume:9 | Issue:1 (22) Page(s): 72-76

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Title

ISOLATION OF ESCHERICHIA COLI O157:H7 FROM GROUND BEEF SAMPLES COLLECTED FROM BEEF MARKETS, USING CONVENTIONAL CULTURE AND POLYMERASE CHAIN REACTION IN MASHHAD, NORTHEASTERN IRAN

Author(s)

JAMSHIDI ABD ELAH | BASAMI M.R. | RASOULI MARYAM | Issue Writer Certificate

Keywords

ESCHERICHIA COLI O157:H7Q3

CULTURE METHODQ3

POLYMERASE CHAIN REACTIONQ3

Abstract

ESCHERICHIA COLI O157:H7 is an important human pathogen causing haemorrhagic colitis, haemolytic-uraemic syndrome and thrombotic thrombocytopenic purpura. In this study, 100 ground beef samples were collected randomly from beef markets in June 2004. For isolation of the bacteria, samples were firstly enriched in modified trypticase soy broth, followed by plating onto sorbitol MacConkey agar supplemented with cefixime and potassium tellurite. Consequently, the suspected non-sorbitol fermenting (NSF) colonies were confirmed by biochemical tests and employed for POLYMERASE CHAIN REACTION (PCR) assay, using primers specific for O157 and H7 antigens gene. In this study, 7 NSF E. coli colonies were isolated; in PCR assay only one of them confirmed as E. coli O157:H7. The PCR assay employed in this study may be a possible alternative to immunological assays which detects somatic and flagellar antigens.

Cites

PREVALENCE AND DISTRIBUTION OF THE STX1, STX2 GENES IN SHIGA TOXIN PRODUCING E. COLI (STEC) ISOLATES FROM CATTLE

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APA: Copy

JAMSHIDI, ABD ELAH, BASAMI, M.R., & RASOULI, MARYAM. (2008). ISOLATION OF ESCHERICHIA COLI O157:H7 FROM GROUND BEEF SAMPLES COLLECTED FROM BEEF MARKETS, USING CONVENTIONAL CULTURE AND POLYMERASE CHAIN REACTION IN MASHHAD, NORTHEASTERN IRAN. IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR), 9(1 (22)), 72-76. SID. https://sid.ir/paper/85600/en

Vancouver: Copy

JAMSHIDI ABD ELAH, BASAMI M.R., RASOULI MARYAM. ISOLATION OF ESCHERICHIA COLI O157:H7 FROM GROUND BEEF SAMPLES COLLECTED FROM BEEF MARKETS, USING CONVENTIONAL CULTURE AND POLYMERASE CHAIN REACTION IN MASHHAD, NORTHEASTERN IRAN. IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR)[Internet]. 2008;9(1 (22)):72-76. Available from: https://sid.ir/paper/85600/en

IEEE: Copy

ABD ELAH JAMSHIDI, M.R. BASAMI, and MARYAM RASOULI, “ISOLATION OF ESCHERICHIA COLI O157:H7 FROM GROUND BEEF SAMPLES COLLECTED FROM BEEF MARKETS, USING CONVENTIONAL CULTURE AND POLYMERASE CHAIN REACTION IN MASHHAD, NORTHEASTERN IRAN,” IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR), vol. 9, no. 1 (22), pp. 72–76, 2008, [Online]. Available: https://sid.ir/paper/85600/en

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