MOLECULAR IDENTIFICATION OF ANAPLASMOSIS IN GOATS USING A NEW PCR-RFLP METHOD

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

AHMADI HAMEDANI M.; KHAKI Z.; RAHBARI S.; KAZEMI BAHRAM; BANDEHPOUR M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR) Year:2009 | Volume:10 | Issue:4 (29) Page(s): 367-372

Download Full-Text

Persian Verion

View:

1,110

Download:

423

Cites:

Information Journal Paper

Title

MOLECULAR IDENTIFICATION OF ANAPLASMOSIS IN GOATS USING A NEW PCR-RFLP METHOD

Author(s)

Keywords

ANAPLASMAQ1

GOATQ3

IRANQ2

PCR-RFLPQ2

Abstract

In this study blood samples were collected from 193 GOATs in north and northeastern IRAN with the aim to develop a PCR-RFLP assay, as a specific and sensitive diagnostic tool enabling direct and concurrent identification of two ANAPLASMA species (A. ovis, A. marginale) in GOATs. A polymerase chain reaction (PCR) for amplification of a fragment of the major surface protein 4 (msp4) gene from A. ovis and A. marginale was developed. The results revealed that 123 out of 193 blood samples were positive for ANAPLASMA spp. infection. All 43 positive samples detected by microscopic examination were approved as positive by PCR, whereas no rickettsials were seen through light microscopy in the other 80 PCR positive cases. All positive samples were A. ovis as confirmed by restriction fragments length polymorphism (RFLP) method. Our results showed that PCR-RFLP of the msp4 gene could be a useful method for the detection of A. ovis in GOATs.

Cites

No record.

References

No record.

Cite

APA: Copy

AHMADI HAMEDANI, M., KHAKI, Z., RAHBARI, S., KAZEMI, BAHRAM, & BANDEHPOUR, M.. (2009). MOLECULAR IDENTIFICATION OF ANAPLASMOSIS IN GOATS USING A NEW PCR-RFLP METHOD. IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR), 10(4 (29)), 367-372. SID. https://sid.ir/paper/85698/en

Vancouver: Copy

AHMADI HAMEDANI M., KHAKI Z., RAHBARI S., KAZEMI BAHRAM, BANDEHPOUR M.. MOLECULAR IDENTIFICATION OF ANAPLASMOSIS IN GOATS USING A NEW PCR-RFLP METHOD. IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR)[Internet]. 2009;10(4 (29)):367-372. Available from: https://sid.ir/paper/85698/en

IEEE: Copy

M. AHMADI HAMEDANI, Z. KHAKI, S. RAHBARI, BAHRAM KAZEMI, and M. BANDEHPOUR, “MOLECULAR IDENTIFICATION OF ANAPLASMOSIS IN GOATS USING A NEW PCR-RFLP METHOD,” IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR), vol. 10, no. 4 (29), pp. 367–372, 2009, [Online]. Available: https://sid.ir/paper/85698/en

Related Journal Papers