SHORT PAPER: GENE DISRUPTION IN SALMONELLA TYPHIMURIM BY MODIFIED λ RED DISRUPTION SYSTEM

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AHANI AZARI A.; ZAHRAEI SALEHI T.; NAYERI FASAEI B.; ALEBOUYEH M.

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Paper Information

Journal: IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR) Year:2015 | Volume:16 | Issue:3 (52) Page(s): 301-305

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Information Journal Paper

Title

SHORT PAPER: GENE DISRUPTION IN SALMONELLA TYPHIMURIM BY MODIFIED λ RED DISRUPTION SYSTEM

Author(s)

AHANI AZARI A. | ZAHRAEI SALEHI T. | NAYERI FASAEI B. | ALEBOUYEH M. | Issue Writer Certificate

Keywords

GENE DISRUPTIONQ2

KANAMYCIN CASSETTEQ2

λ RED DISRUPTION SYSTEMQ2

SALMONELLA TYPHIMURIUMQ2

SOEING PCR METHODQ2

Abstract

There are many techniques to knock out directed genes in bacteria, some of which have been described in Salmonella species. In this study, a combination of SOEING PCR METHOD and the l Red disruption system were used to disrupt phoP gene in wild type and standard strains of SALMONELLA TYPHIMURIUM. Three standards PCR and one fusion PCR reactions were performed to construct a linear DNA including upstream and downstream of phoP gene and KANAMYCIN CASSETTE. As a template plasmid, we used pKD4 which carries kanamycin gene flanked by FRT (FLP recognition target) sites. The resulting construct was electroporated into prepared competent cells of S. typhimurium. The transformants colonies related to the standard strain appeared on the LB-Km-agar plates after incubation, but there was no colony on LB-Km-agar plates corresponding to the wild type strain. The failure in transformation of the wild type strain may be because of inflexibility of the l Red disruption system in this strain or its unique restriction-modification system. However, by this construct we are able to generate phoP mutant in many of the Salmonella species due to high homology of the phoP gene which exists in different species.

Cites

IN VITRO SUSCEPTIBILITY STUDY OF SALMONELLA TYPHIMURIUM MUTANT TO STRESS FACTORS

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APA: Copy

AHANI AZARI, A., ZAHRAEI SALEHI, T., NAYERI FASAEI, B., & ALEBOUYEH, M.. (2015). SHORT PAPER: GENE DISRUPTION IN SALMONELLA TYPHIMURIM BY MODIFIED λ RED DISRUPTION SYSTEM. IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR), 16(3 (52)), 301-305. SID. https://sid.ir/paper/85739/en

Vancouver: Copy

AHANI AZARI A., ZAHRAEI SALEHI T., NAYERI FASAEI B., ALEBOUYEH M.. SHORT PAPER: GENE DISRUPTION IN SALMONELLA TYPHIMURIM BY MODIFIED λ RED DISRUPTION SYSTEM. IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR)[Internet]. 2015;16(3 (52)):301-305. Available from: https://sid.ir/paper/85739/en

IEEE: Copy

A. AHANI AZARI, T. ZAHRAEI SALEHI, B. NAYERI FASAEI, and M. ALEBOUYEH, “SHORT PAPER: GENE DISRUPTION IN SALMONELLA TYPHIMURIM BY MODIFIED λ RED DISRUPTION SYSTEM,” IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR), vol. 16, no. 3 (52), pp. 301–305, 2015, [Online]. Available: https://sid.ir/paper/85739/en

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