ANALYSIS OF CHROMATIN INTEGRITY AND DNA DAMAGE OF BUFFALO SPERMATOZOA

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MAHMOUD K.GH.M.; EL SOKARY A.A.E.; ABDEL GHAFFAR A.E.; ABOU EL ROOS M.E.A.; AHMED Y.F.

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Journal: IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR) Year:2015 | Volume:16 | Issue:2 (51) Page(s): 161-166

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Title

ANALYSIS OF CHROMATIN INTEGRITY AND DNA DAMAGE OF BUFFALO SPERMATOZOA

Author(s)

Keywords

BUFFALO BULLQ2

CHROMATIN INTEGRITYQ2

DNA DAMAGEQ2

SEMEN QUALITYQ2

Abstract

This study was conducted to determine CHROMATIN INTEGRITY and DNA DAMAGE by DNA electrophoresis and comet assays of buffalo fresh and frozen semen. Semen samples were collected from four BUFFALO BULLs and evaluated after freezing for semen motility, viability, sperm abnormalities, CHROMATIN INTEGRITY and DNA DAMAGE. A significant variation was found in semen parameters after thawing. Highly significant differences (P<0.001) in CHROMATIN INTEGRITY were observed between fresh and frozen semen. For the fresh semen, there was no significant difference between the bulls for CHROMATIN INTEGRITY; however, a significant variation (P<0.05) was detected in their frozen semen. No DNA fragmentation was observed by agarose gel electrophoresis. The percentage of sperm with damaged DNA detected by comet assay differed significantly between fresh and frozen semen. A significant negative correlation was recorded between motility and DNA DAMAGE (r=-0.68, P<0.05). Sperm abnormalities and DNA fragmentation were significantly positively correlated (r=0.59, P<0.05). In conclusion, DNA DAMAGE evaluation can provide reassurance about genomic normalcy and guide the development of improved methods of selecting spermatozoa with intact DNA to be used in artificial insemination.

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APA: Copy

MAHMOUD, K.GH.M., EL SOKARY, A.A.E., ABDEL GHAFFAR, A.E., ABOU EL ROOS, M.E.A., & AHMED, Y.F.. (2015). ANALYSIS OF CHROMATIN INTEGRITY AND DNA DAMAGE OF BUFFALO SPERMATOZOA. IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR), 16(2 (51)), 161-166. SID. https://sid.ir/paper/85769/en

Vancouver: Copy

MAHMOUD K.GH.M., EL SOKARY A.A.E., ABDEL GHAFFAR A.E., ABOU EL ROOS M.E.A., AHMED Y.F.. ANALYSIS OF CHROMATIN INTEGRITY AND DNA DAMAGE OF BUFFALO SPERMATOZOA. IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR)[Internet]. 2015;16(2 (51)):161-166. Available from: https://sid.ir/paper/85769/en

IEEE: Copy

K.GH.M. MAHMOUD, A.A.E. EL SOKARY, A.E. ABDEL GHAFFAR, M.E.A. ABOU EL ROOS, and Y.F. AHMED, “ANALYSIS OF CHROMATIN INTEGRITY AND DNA DAMAGE OF BUFFALO SPERMATOZOA,” IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR), vol. 16, no. 2 (51), pp. 161–166, 2015, [Online]. Available: https://sid.ir/paper/85769/en

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