DETECTION OF HEPATITIS G VIRUS (GBV-C), IN CHRONIC HEPATITIS C PATIENT,BY RT-NESTED PCR; CASE REPORT

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GHANBARI REZA; RAVANSHAD M.; HOSSEINI S.Y.; SHAHZAMANI K.

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Journal: GOVARESH JOURNAL Year:2009 | Volume:14 | Issue:3 (SN 68) Page(s): 153-160

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Title

DETECTION OF HEPATITIS G VIRUS (GBV-C), IN CHRONIC HEPATITIS C PATIENT,BY RT-NESTED PCR; CASE REPORT

Author(s)

GHANBARI REZA | RAVANSHAD M. | HOSSEINI S.Y. | SHAHZAMANI K. | Issue Writer Certificate

Keywords

NESTED PCRQ3

RT-PCRQ3

HEPATITIS G VIRUSQ3

5'-UTRQ3

Abstract

Background: HEPATITIS G VIRUS (GBV-C) is a single strand RNA positive virus and is a member of flaviviridae family. Infection with the virus is prevalent add has worldwide distribution. Accurate diagnosis of the virus very important, because of co-infection with other important viruses like HCV, HBV and HIV, it may influence on pathogenesis, disease progression and response to viral therapy.Materials and Methods: In the current investigation a sensitive and accurate and detection of 5'-UTR sequences of HEPATITIS G VIRUS (GBV-C) was developed and applied. First genome of the virus was extracted and then, by Reverse Transcription method, cDNA was synthesized. Then, by use of a double stage PCR and two pairs of specific primers, the specified region of the virus genome was amplified and the final product was visualized on agarose gel electrophoresis.Results: Based on five positive samples, the method was developed and optimized. Developed method, sera samples from chronic HCV infected patients, (GBV-C). Finally, it was shown that 31 (43.6%) patients were positive for HEPATITIS G VIRUS.Conclusion: Based in the results in the current study, it was shown that the developed assay has performance for diagnosis of HEPATITIS G VIRUS infection. Also it was concluded that the infection rate among chronic HCV infected patients is high

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APA: Copy

GHANBARI, REZA, RAVANSHAD, M., HOSSEINI, S.Y., & SHAHZAMANI, K.. (2009). DETECTION OF HEPATITIS G VIRUS (GBV-C), IN CHRONIC HEPATITIS C PATIENT,BY RT-NESTED PCR; CASE REPORT. GOVARESH JOURNAL, 14(3 (SN 68)), 153-160. SID. https://sid.ir/paper/86206/en

Vancouver: Copy

GHANBARI REZA, RAVANSHAD M., HOSSEINI S.Y., SHAHZAMANI K.. DETECTION OF HEPATITIS G VIRUS (GBV-C), IN CHRONIC HEPATITIS C PATIENT,BY RT-NESTED PCR; CASE REPORT. GOVARESH JOURNAL[Internet]. 2009;14(3 (SN 68)):153-160. Available from: https://sid.ir/paper/86206/en

IEEE: Copy

REZA GHANBARI, M. RAVANSHAD, S.Y. HOSSEINI, and K. SHAHZAMANI, “DETECTION OF HEPATITIS G VIRUS (GBV-C), IN CHRONIC HEPATITIS C PATIENT,BY RT-NESTED PCR; CASE REPORT,” GOVARESH JOURNAL, vol. 14, no. 3 (SN 68), pp. 153–160, 2009, [Online]. Available: https://sid.ir/paper/86206/en

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