DESIGN AND CONSTRUCTION OF FOUR RECOMBINANT PLASMID VECTORS CONTAINING CHITINASE, GLUCANASE AND BT GENES, SUITABLE FOR PLANT TRANSFORMATION

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MOHSENPOUR M.; BABAEIAN JELOUDAR N.A.; TOUHIDFARD MASOUD; HABASHI A.A.

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Journal: Journal of Agricultural Sciences and Natural Resources Year:2008 | Volume:15 | Issue:4 Page(s): 69-80

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Title

DESIGN AND CONSTRUCTION OF FOUR RECOMBINANT PLASMID VECTORS CONTAINING CHITINASE, GLUCANASE AND BT GENES, SUITABLE FOR PLANT TRANSFORMATION

Author(s)

MOHSENPOUR M. | BABAEIAN JELOUDAR N.A. | TOUHIDFARD MASOUD | HABASHI A.A. | Issue Writer Certificate

Keywords

BTQ3

CHITINASEQ3

CLONINGQ2

GLUCANASEQ3

RECOMBINANT PLASMIDQ3

Abstract

Four plasmid vectors that are suitable for plant transformation were constructed by preparation of gene cassettes for b-1, 3-glucanase from barley, CHITINASE from bean and cryIA (b) from Bacillus thuringiensis (BT). Each of these genes was cloned under the control of the CaMV35S promoter and the Nos terminator in pBI121 binary vector. CHITINASE and b-1,3- GLUCANASE genes that encode antifungal proteins, can hydrolyzes b-1,3- glucans and chitin, respectively, which are major components of the cell walls of many pathogenic fungi and increase plants resistance against fungal infection. CryIA (b) gene also encodes an insecticide protein that is generally active against lepidopteran insects. pBI-Glu and pBI-Chi RECOMBINANT PLASMID vectors were constructed via CLONING of b-1,3- GLUCANASE and CHITINASE genes, respectively, instead of the gus gene in T-DNA region of pBI121 vector. Construction of pBI-ChiGlu RECOMBINANT PLASMID vector was performed by means of CLONING both of the complete CHITINASE and GLUCANASE gene cassettes in pBI121 vector, with the intention of production synergistic effects against fungal infection. pBI-ChiBT RECOMBINANT PLASMID vector containing both of the complete CHITINASE and BT gene cassettes was also constructed in order to simultaneous plants resistance to fungal and pest pathogens in a single transformation event. These four RECOMBINANT PLASMID vectors can be useful for plant transformation via Agrobacterium and other transformation approaches such as biolistic.

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APA: Copy

MOHSENPOUR, M., BABAEIAN JELOUDAR, N.A., TOUHIDFARD, MASOUD, & HABASHI, A.A.. (2008). DESIGN AND CONSTRUCTION OF FOUR RECOMBINANT PLASMID VECTORS CONTAINING CHITINASE, GLUCANASE AND BT GENES, SUITABLE FOR PLANT TRANSFORMATION. JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES, 15(4), 69-80. SID. https://sid.ir/paper/8895/en

Vancouver: Copy

MOHSENPOUR M., BABAEIAN JELOUDAR N.A., TOUHIDFARD MASOUD, HABASHI A.A.. DESIGN AND CONSTRUCTION OF FOUR RECOMBINANT PLASMID VECTORS CONTAINING CHITINASE, GLUCANASE AND BT GENES, SUITABLE FOR PLANT TRANSFORMATION. JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES[Internet]. 2008;15(4):69-80. Available from: https://sid.ir/paper/8895/en

IEEE: Copy

M. MOHSENPOUR, N.A. BABAEIAN JELOUDAR, MASOUD TOUHIDFARD, and A.A. HABASHI, “DESIGN AND CONSTRUCTION OF FOUR RECOMBINANT PLASMID VECTORS CONTAINING CHITINASE, GLUCANASE AND BT GENES, SUITABLE FOR PLANT TRANSFORMATION,” JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES, vol. 15, no. 4, pp. 69–80, 2008, [Online]. Available: https://sid.ir/paper/8895/en

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