INVESTIGATING THE LEVEL OF ANTIBIOTIC RESISTANCE AND DETECTION OF BETALACTAMASE OF BLATEM HIGH FREQUENCY IN THE ESBLS PRODUCING E. COLI ISOLATED IN RASHT

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

HAGHIGHATPANAH M.; AMIRMOZAFARI N.; FAEZI M.; SHENAGARI M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Ilam University of Medical Sciences Year:2014 | Volume:22 | Issue:SUPPLEMENT Page(s): 180-189

Download Full-Text

Persian Verion

View:

1,489

Download:

Cites:

Information Journal Paper

Title

INVESTIGATING THE LEVEL OF ANTIBIOTIC RESISTANCE AND DETECTION OF BETALACTAMASE OF BLATEM HIGH FREQUENCY IN THE ESBLS PRODUCING E. COLI ISOLATED IN RASHT

Author(s)

HAGHIGHATPANAH M. | AMIRMOZAFARI N. | FAEZI M. | SHENAGARI M. | Issue Writer Certificate

Keywords

E. COLIQ2

ESBLSQ1

BLATEMQ2

Abstract

Introduction: E.coli is one of the most frequent causes of nosocomial infections. Antimicrobial resistance leads to failure in treatment of hospital infections caused by E. COLI. Production of extended-Spectrum Beta- lactamases (ESBLs) is one of the causes of antibiotic resistance in these bacteria. These enzymes are predominantly plasmid mediated and are derived from TEM and SHV type enzymes. The aim of this study was to investigate the frequency of blaTEM genes in ESBL-producing E.coli strains isolated from admitted patients in six hospitals in Rasht, Iran.Materials & Methods: Among all clinical samples, 160 E. COLI were taken from various samples of hospitalized patients after diagnosis of E.coli. Antibiotic resistance was surveyed by Kirby-Bauer method. For resistant isolates, double disk phenotypic confirmatory test was carried out in order to diagnosis ESBL-producing strains. Then, DNA extracted and blaTEM genes were detected using PCR from ESBL-producing strains.Finding: Among the160clinical isolates of E.coli which were collected, the maximum resistance to amoxicillin was observed in all strains, all were susceptible to Imipenem, and 51.9% of strains were ESBL positive, and 27 strains (32.5%) of blaTEM genes were observed using PCR. Discussion &Conclusion: In this study, more than 50% were detected as ESBL-producing strains which mean that more than half of the isolates were blaTEM positive. Comparing these results with other studies in this field shows a direct relation between development of resistance to beta-lactam antibiotics and genes transfer such as blaTEM.

Cites

EVALUATION OF ESBL GENES OF BLATEM, BLASHV, BLAOXA AND AMINOGLYCOSIDE GENE OF AADA IN ESCHERICHIA COLI STRAINS ISOLATED IN TEHRAN AND DETERMINATION OF ANTIMICROBIAL RESISTANCE

References

No record.

Cite

APA: Copy

HAGHIGHATPANAH, M., AMIRMOZAFARI, N., FAEZI, M., & SHENAGARI, M.. (2014). INVESTIGATING THE LEVEL OF ANTIBIOTIC RESISTANCE AND DETECTION OF BETALACTAMASE OF BLA_TEM HIGH FREQUENCY IN THE ESBLS PRODUCING E. COLI ISOLATED IN RASHT. JOURNAL OF ILAM UNIVERSITY OF MEDICAL SCIENCES, 22(SUPPLEMENT), 180-189. SID. https://sid.ir/paper/90258/en

Vancouver: Copy

HAGHIGHATPANAH M., AMIRMOZAFARI N., FAEZI M., SHENAGARI M.. INVESTIGATING THE LEVEL OF ANTIBIOTIC RESISTANCE AND DETECTION OF BETALACTAMASE OF BLA_TEM HIGH FREQUENCY IN THE ESBLS PRODUCING E. COLI ISOLATED IN RASHT. JOURNAL OF ILAM UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2014;22(SUPPLEMENT):180-189. Available from: https://sid.ir/paper/90258/en

IEEE: Copy

M. HAGHIGHATPANAH, N. AMIRMOZAFARI, M. FAEZI, and M. SHENAGARI, “INVESTIGATING THE LEVEL OF ANTIBIOTIC RESISTANCE AND DETECTION OF BETALACTAMASE OF BLA_TEM HIGH FREQUENCY IN THE ESBLS PRODUCING E. COLI ISOLATED IN RASHT,” JOURNAL OF ILAM UNIVERSITY OF MEDICAL SCIENCES, vol. 22, no. SUPPLEMENT, pp. 180–189, 2014, [Online]. Available: https://sid.ir/paper/90258/en

Related Journal Papers