INVESTIGATION OF HBV-DNA IN INDIVIDUALS WITH SERONEGATIVE HBSAG INDICATORS BY REAL-TIME PCR IN ILAM (WESTERN IRAN)

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GHOLAMI PARIZAD E.; GHOLAMI PARIZAD E.S.; SADEGHIFARD N.; AMIRI ANDI S.A.

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Journal: JOURNAL OF ILAM UNIVERSITY OF MEDICAL SCIENCES Year:2011 | Volume:19 | Issue:1 Page(s): 32-38

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Title

INVESTIGATION OF HBV-DNA IN INDIVIDUALS WITH SERONEGATIVE HBSAG INDICATORS BY REAL-TIME PCR IN ILAM (WESTERN IRAN)

Author(s)

GHOLAMI PARIZAD E. | GHOLAMI PARIZAD E.S. | SADEGHIFARD N. | AMIRI ANDI S.A. | Issue Writer Certificate

Keywords

HEPATITIS B NEGATIVE HBSAGQ2

SERUMQ3

ELISAQ2

REAL-TIME PCRQ2

Abstract

Introduction: Hepatitis B infection is a considerable health problem throughout the world involving more than 2 billions people. It is estimated that over 370 million people are chronic carriers of HBV worldwide. It has been revealed that 3% of Iranian populations are chronic carriers of this virus. The current study aimed to detect HBV DNA amongst some HBsAg negative individuals as chronic carriers of HBV using sera as a source of infection in Ilam (western Iran).Materials & Methods: In a case–control study, the viral DNA in sera of 70 healthy individuals as case group was evaluated during 2009-10. Samples were tested using PCR and Real Time PCR while for some immunological evaluations ELISA was performed using sera of healthy people.Findings: All the 70 individuals (100%) in control group were HBsAg positive, while in the case group, only 2 people (2.8%) were HBsAg positive. 3 individuals in case group were positive using PCR and Real Time PCR, indicating that about 7% of healthy individuals were chronic carriers of HBV.Discussion & Conclusion: It was concluded that seronegative HBsAg indicating individuals can be carriers of HBV-DNA. Therefore, ELISA test of HBsAg can not alone be a reliable approach to diagnose hepatitis B in people without or with chronic H.B. As a result, Medical doctors are expected to apply other diagnostic methods such as molecular approaches to detect the disease.

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APA: Copy

GHOLAMI PARIZAD, E., GHOLAMI PARIZAD, E.S., SADEGHIFARD, N., & AMIRI ANDI, S.A.. (2011). INVESTIGATION OF HBV-DNA IN INDIVIDUALS WITH SERONEGATIVE HBSAG INDICATORS BY REAL-TIME PCR IN ILAM (WESTERN IRAN). JOURNAL OF ILAM UNIVERSITY OF MEDICAL SCIENCES, 19(1), 32-38. SID. https://sid.ir/paper/90337/en

Vancouver: Copy

GHOLAMI PARIZAD E., GHOLAMI PARIZAD E.S., SADEGHIFARD N., AMIRI ANDI S.A.. INVESTIGATION OF HBV-DNA IN INDIVIDUALS WITH SERONEGATIVE HBSAG INDICATORS BY REAL-TIME PCR IN ILAM (WESTERN IRAN). JOURNAL OF ILAM UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2011;19(1):32-38. Available from: https://sid.ir/paper/90337/en

IEEE: Copy

E. GHOLAMI PARIZAD, E.S. GHOLAMI PARIZAD, N. SADEGHIFARD, and S.A. AMIRI ANDI, “INVESTIGATION OF HBV-DNA IN INDIVIDUALS WITH SERONEGATIVE HBSAG INDICATORS BY REAL-TIME PCR IN ILAM (WESTERN IRAN),” JOURNAL OF ILAM UNIVERSITY OF MEDICAL SCIENCES, vol. 19, no. 1, pp. 32–38, 2011, [Online]. Available: https://sid.ir/paper/90337/en

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