IN VITRO ESTABLISHMENT OF THREE OLIVE CULTIVARS (OLEA EUROPAEA L.)

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KIANI FERIZ M.; ZAMANI Z.; EBADI A.

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Journal: Journal of Agricultural Sciences and Natural Resources Year:2005 | Volume:12 | Issue:4 Page(s): 29-37

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Title

IN VITRO ESTABLISHMENT OF THREE OLIVE CULTIVARS (OLEA EUROPAEA L.)

Author(s)

KIANI FERIZ M. | ZAMANI Z. | EBADI A. | Issue Writer Certificate

Keywords

MICROPROPAGATIONQ4

ESTABLISHMENT AND OLIVEQ4

Abstract

Tissue culture systems provide a high efficiency for mass propagation of desired disease-free plants as well as genetic improvement. Considering these facts and the importance of olive crop, some experiments were carried out to investigate the in vitro establishment of three olive cultivars. For this purpose, single nodes were taken from mature container grown 'Manzanilla', 'Dezfoul' and 'Zard' olive cultivars. To find out the best disinfection method, different concentrations of NaOC1 and HgCl2 were used. Three sampling times i.e. 4th of February, 5th of May and 6th of August and three culture media i.e. OM, MS and MS with half strength of macro elements (1/2MS) were compared to optimize shoot establishment. The most important contamination agent was diagnosed and the controlling effect of Cefotaxime antibiotic was evaluated. Furthermore, the effects of two cytokines: Benzyladenine (BA) and Zeatin on establishment were evaluated. The Results showed that treated explants with 0.1% HgCl2 for 3 min. followed by three times rinsing with sterile distilled water was the most appropriate disinfection method. Explants, which were taken on 6thof August and grown in 1/2 MS medium, gave the best results. The main contaminating agent was Pseudomonas bacterium which was controlled by Cefotaxime at 100 mg/I. adding 0.5 mg/l BA to 1/2 MS medium improved in vitro establishment of olive explants.

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APA: Copy

KIANI FERIZ, M., ZAMANI, Z., & EBADI, A.. (2005). IN VITRO ESTABLISHMENT OF THREE OLIVE CULTIVARS (OLEA EUROPAEA L.). JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES, 12(4), 29-37. SID. https://sid.ir/paper/9249/en

Vancouver: Copy

KIANI FERIZ M., ZAMANI Z., EBADI A.. IN VITRO ESTABLISHMENT OF THREE OLIVE CULTIVARS (OLEA EUROPAEA L.). JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES[Internet]. 2005;12(4):29-37. Available from: https://sid.ir/paper/9249/en

IEEE: Copy

M. KIANI FERIZ, Z. ZAMANI, and A. EBADI, “IN VITRO ESTABLISHMENT OF THREE OLIVE CULTIVARS (OLEA EUROPAEA L.),” JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES, vol. 12, no. 4, pp. 29–37, 2005, [Online]. Available: https://sid.ir/paper/9249/en

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